Active Recombinant Human IL1B
Cat.No. : | IL1B-339H |
Product Overview : | Human IL1B (P01584, 117 a.a.-269 a.a.) partial recombinant protein expressed in Escherichia coli. |
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- Gene Information
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Cat. No. : | IL1B-339H |
Description : | Interleukin-1 beta (IL-1μ) also known as catabolin, is a cytokine protein that in humans is encoded by the IL1B gene. IL-1μ precursor is cleaved by caspase 1 (interleukin 1 beta convertase). Cytosolic thiol protease cleaves the product to form mature IL-1μ. IL-1μ is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. |
Sequence : | APVR SLNCTLRDSQ QKSLVMSGPY ELKALHLQGQ DMEQQVVFSM SFVQGEESND KIPVALGLKE KNLYLSCVLK DDKPTLQLES VDPKNYPKKK MEKRFVFNKI EINNKLEFES AQFPNWYIST SQAENMPVFL GGTKGGQDIT DFTMQFVSS |
Theoretical MW (kDa) : | 17 |
Form : | Lyophilized |
Preparation Method : | Escherichia coli expression system |
Purity : | > 97% by SDS-PAGE and HPLC |
Endotoxin Level : | < 0.1 ng/ug (1 EU/ug) |
Activity : | The ED50 as determined by the dose-dependent proliferation of murine D10S cells, was found to be μ2.0 pg/ml, corresponding to a specific activity of μ2 x 109 units/mg. |
Application : | SDS-PAGE |
Storage Buffer : | Lyophilized from PBS, pH 7.0 |
Storage : | Store at -20°C on dry atmosphere for 2 years. After reconstitution with deionized water, store at 4°C for 1 month or store at -20°C for 6 months. Aliquot to avoid repeated freezing and thawing. |
Unitprot ID : | P01584 |
Pathways : | Alzheimer"s disease; Apoptosis; Cytokine-cytokine receptor interaction; Hematopoietic cell lineage; MAPK signaling pathway; Toll-like receptor signaling pathway; Type I diabetes mellitus. |
Gene Name : | IL1B interleukin 1, beta [ Homo sapiens ] |
Official Symbol : | IL1B |
Synonyms : | IL-1; IL1F2; IL1-BETA; Catabolin; IL-1B; pro-interleukin-1-beta; Interleukin-1 beta precursor; interleukin 1, beta; preinterleukin 1 beta; IL1B_HUMAN. |
Gene ID : | 3553 |
mRNA Refseq : | NM_000576 |
Protein Refseq : | NP_000567 |
MIM : | 147720 |
Chromosome Location : | 2q14 |
Function : | interleukin-1 receptor binding; protein binding; signal transducer activity |
Products Types
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IL1B-667R | Active Recombinant Rabbit IL1B protein(Ala117-Ser268) | +Inquiry |
Il1b-101M | Active Recombinant Mouse Il1b Protein | +Inquiry |
Il1b-161M | Recombinant Active Mouse IL1B Protein, His-tagged(C-ter) | +Inquiry |
◆ Lysates | ||
IL1B-853HCL | Recombinant Human IL1B cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionInflammasome assembly initiated by IL-1β is deciphered using techniques like co-immunoprecipitation, proximity ligation assays, and super-resolution microscopy.
IL-1β's priming of immune cells is dissected through flow cytometry, RNA sequencing, and chromatin immunoprecipitation, revealing its epigenetic and transcriptional effects.
Mass spectrometry-based phosphoproteomics deciphers IL-1β's post-translational modifications, revealing regulatory sites crucial for its activation and signaling.
Bioinformatics tools integrate multi-omics data to provide a comprehensive understanding of IL-1β's roles, revealing its intricate contributions to health and disease.
ChIP-seq and DNA methylation profiling uncover IL-1β's epigenetic impact on target genes, shedding light on its role in shaping chronic inflammatory states.
IL-1β's interactions with downstream effectors are unveiled using dual-luciferase reporter assays, surface plasmon resonance, and co-crystallization studies.
Network analysis integrates omics data to unravel IL-1β's intricate cross-talk with other cytokines, mapping out complex regulatory loops in inflammation.
IL-1β's impact on the tumor microenvironment is studied using intravital microscopy, 3D culture models, and single-cell RNA sequencing to capture dynamic cell behavior.
CRISPR-Cas9 screening identifies IL-1β's non-canonical signaling mediators through functional genomics, revealing alternative pathways in cellular responses.
Single-cell RNA sequencing unveils IL-1β's context-specific responses, exposing cell type-specific signaling and gene expression patterns.
Customer Reviews (3)
Write a reviewEffective in inflammation studies.
High specificity.
Suitable for cytokine assays.
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