Active Recombinant Human IL1RL1, His-tagged, Biotinylated

• Cat.No.: IL1RL1-629H
• Product Overview: The recombinant human IL1RL1 ECD is expressed as a 320 amino acid protein consisting of Lys19 - Ser328 region of IL1RL1/ST2 (UniProt accession #Q01638) and a C-terminal His-tag.

Specification

• Source: Human cells
• Species: Human
• Tag: His
• Form: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule).
• Bio-activity: Immobilized IL1RL1 binds human IL33 in a functional ELISA. Blocks IL33-dependent signaling activity in helper T cells.
• Molecular Mass: Calculated molecular mass (kDa): 36.3; Estimated by SDS-PAGE under reducing condition (kDa): 60-70 (probably due to glycosylation).
• AA Sequence: KFSKQSWGLENEALIVRCPRQGKPS YTVDWYYSQTNKSIPTQERNRVFAS GQLLKFLPAAVADSGIYTCIVRSP TFNRTGYANVTIYKKQSDCNVPDYL MYSTVSGSEKNSKIYCPTIDLYNWT APLEWFKNCQALQGSRYRAHKSFL VIDNVMTEDAGDYTCKFIHNENGAN YSVTATRSFTVKDEQGFSLFPVIGA PAQNEIKEVEIGKNANLTCSACFG KGTQFLAAVLWQLNGTKITDFGEPR IQQEEGQNQSFSNGLACLDMVLRIA DVKEEDLLLQYDCLALNLHGLRR HTVRLSRKNPIDHHSTGHHHHHHHH
• Endotoxin: <0.1 eu per 1 μg of purified recombinant protein determined by the lal>
• Purity: >90% judged by SDS-PAGE under reducing condition
• Storage: The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks. Avoid repeated freeze-thaw cycles.
• Protein length: Lys19-Ser328

Gene Information

• Gene Name: IL1RL1 interleukin 1 receptor-like 1 [ Homo sapiens ]
• Official Symbol: IL1RL1
• Synonyms: IL1RL1; interleukin 1 receptor-like 1; interleukin-1 receptor-like 1; DER4; FIT 1; homolog of mouse growth stimulation expressed; IL33R; ST2; ST2L; ST2V; T1; growth stimulation-expressed; interleukin 1 receptor-related protein; homolog of mouse growth stimulation-expressed; FIT-1; MGC32623
• Gene ID: 9173
• mRNA Refseq: NM_003856
• Protein Refseq: NP_003847
• MIM: 601203
• UniProt ID: Q01638
• Chromosome Location: 2q12
• Function: cytokine receptor activity; interleukin-1 receptor activity; interleukin-33 binding; protein binding; receptor activity; receptor signaling protein activity

Reviews

07/25/2019

High specificity in ELISA.

01/30/2017

Suitable for flow cytometry.

07/13/2016

Effective in allergy models.

Q&As

Are there any splice variants of IL1RL1?

IL1RL1 has multiple splice variants that differ in signaling capabilities.

What is the role of IL1RL1 in allergic responses?

IL1RL1 suppresses allergic responses by inhibiting mast cell activation.

Does IL1RL1 have a role in autoimmune diseases?

IL1RL1 is implicated in rheumatoid arthritis.

How does IL1RL1 affect cytokine production?

IL1RL1 increases the production of IL-4 and IL-13.

What is the subcellular localization of IL1RL1?

IL1RL1 is primarily membrane-bound.

Does IL1RL1 influence T-cell differentiation?

IL1RL1 promotes Th2 cell differentiation.

How does IL1RL1 interact with its ligands?

IL1RL1 binds to IL-33 and IL-36 to initiate signaling.

Is IL1RL1 expression altered in any cancers?

IL1RL1 expression is altered in lung and breast cancer.

What post-translational modifications occur on IL1RL1?

IL1RL1 undergoes glycosylation and phosphorylation.

How is IL1RL1 expression regulated in asthmatic conditions?

IL1RL1 expression is upregulated in asthmatic conditions.