Active Recombinant Human IL2RA

• Cat.No.: IL2RA-4951H
• Product Overview: Recombinant Human IL2RA was expressed in insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Non
• Description: The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
• Form: Lyophilized from PBS. Reconstitute with PBS, pH 7.2 to a concentration of 0.1 - 1.0 mg/ml.
• Bio-activity: Determined by its ability to increase the proliferation effect of IL-2 in Mouse CTLL-2 cells. In the presence of 1 ng/ml of recombinant IL-2, the expected ED50 for this effect is between 0.5 - 1.5 ug/ml.
• Molecular Mass: 31 kDa
• AA Sequence: ELCDDDPPEI PHATFKAMAY KEGTMLNCEC KRGFRRIKSG SLYMLCTGNS SHSSWDNQCQ CTSSATRNTT KQVTPQPEEQ KERKTTEMQS PMQPVDQASL PGHCREPPPW ENEATERIYH FVVGQMVYYQ CVQGYRALHR GPAESVCKMT HGKTRWTQPQ LICTGEMETS QFPGEEKPQA SPEGRPESET SCLVTTTDFQ IQTEMAATME TSIFTTEYQ.
• Endotoxin: Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
• Purity: > 98% pure
• Applications: User optimized
• Storage: Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: IL2RA interleukin 2 receptor, alpha [ Homo sapiens (human) ]
• Official Symbol: IL2RA
• Synonyms: IL2RA; interleukin 2 receptor, alpha; IL2R; interleukin-2 receptor subunit alpha; CD25; TAC antigen; IL-2R subunit alpha; IL-2 receptor subunit alpha
• Gene ID: 3559
• mRNA Refseq: NM_000417
• Protein Refseq: NP_000408
• MIM: 147730
• UniProt ID: P01589
• Chromosome Location: 10p15-p14
• Pathway: Arf6 trafficking events; Calcineurin-regulated NFAT-dependent transcription in lymphocytes; Cytokine-cytokine receptor interaction
• Function: drug binding; interleukin-2 binding; interleukin-2 receptor activity