Active Recombinant Human IL4 Protein (His25-Ser153), C-His tagged, Animal-free, Carrier-free

• Cat.No.: IL4-168H
• Product Overview: Recombinant Human IL4 Protein (His25-Ser153) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Interleukin-4 (IL-4) is a key cytokine produced by activated T cells, mast cells, basophils, neutrophils and eosinophils. IL-4 is critical for the development of Th2-mediated responses, which is related to allergy and asthma. It can also regulate B cell responses, including survival, cell proliferation and gene expression. IL-4 also plays fundamental role for B-cell stimulation, including induction of the IgE isotype switch.
• Source: E. coli
• Species: Human
• Tag: C-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce TF-1 cells proliferation. The ED50 for this effect is < 0.2 ng/mL. The specific activity of recombinant human IL-4 is approximately >2.8 × 10⁷ IU/mg.
• Molecular Mass: The protein has a calculated MW of 15.9 kDa.
  The protein migrates as 12 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: His25-Ser153
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 2 weeks under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: IL4 interleukin 4 [ Homo sapiens (human) ]
• Official Symbol: IL4
• Synonyms: IL4; interleukin 4; interleukin-4; B cell growth factor 1; B_cell stimulatory factor 1; BCGF 1; BCGF1; BSF1; IL 4; lymphocyte stimulatory factor 1; MGC79402; binetrakin; pitrakinra; IL-4; BSF-1; BCGF-1
• Gene ID: 3565
• mRNA Refseq: NM_000589
• Protein Refseq: NP_000580
• MIM: 147780
• UniProt ID: P05112