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Active Recombinant Human Interleukin 17F

Cat.No. : IL17F-114H
Product Overview : Recombinant Human Interleukin 17F encoding the human IL-17F protein sequence (containing the signalpeptide sequence, and the mature IL-17F sequence) was expressed in modifiedhuman 293 cells.
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Cat. No. : IL17F-114H
Description : Interleukin-17 (IL-17) is a 155 amino acid protein that is a disulfide linked, homodimeric, secreted glycoprotein with one N-linked glycosylation site. IL-17 is also referred to as IL-17A. Other members include IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. IL-17 binds to the cell surfacer receptor IL-17R. IL-17 is secreted by a subset of T cells called T-helper 17 (Th-17) cells. IL-23 is thought to mediate the production of IL-17 by Th-17 cells.
Source : Human 293 cells.
Amino Acid Sequence : RKIPKVGHTFFQKPESCPPVPGGSM KLDIGIINENQRVSMSRNIESRSTS PWNYTVTWDPNRYPSEVVQAQCRNL GCINAQGKEDISMNSVPIQQETLVV RRKHQGCSVSFQLEKVLVTVGCTCV TPVIHHVQ.
Molecular Mass : Under reducing conditions Apollo IL-17F hcx migrates as a broad band between 17 and 21 kDa on SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified IL-17F polypeptide that has a predicted monomeric molecular mass of 14.9 kDa.
pI : Apollo IL-17F hcx separates into a number of glycoforms with a pI between 6.2 and 9.5 on 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified IL-17F that has a predicted pI of 9.04.
% Carbohydrate : Apollo purified IL-17F hcx consists of 20-35% carbohydrate by weight.
Glycosylation : Apollo IL-17F hcx contains N-linked oligosaccharides.
Purity : >95%, as determined by SDS-PAGE, visualised by Coomassie Brilliant Blue.
Formulation : When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Reconstitution : It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Storage : Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C and longer-term storage of aliquots at -18 to -20°C is recommended. Repeated freeze thawing is not recommended.
Activity : The activity of IL-17F is measured by its ability to induce IL-6 production in NHDF cells. The ED50is typically between 0.04 and 0.1 ng/ml.
Gene Name : IL17F interleukin 17F [ Homo sapiens ]
Synonyms : IL17F; interleukin 17F; ML1; ML-1; IL-17F; cytokine ML-1;Interleukin-24;leukin-17F
Gene ID : 112744
mRNA Refseq : NM_052872
Protein Refseq : NP_443104
UniProt ID : Q96PD4
Chromosome Location : 6p12
MIM : 606496
Function : cytokine activity; cytokine binding; protein homodimerization activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What techniques are used to assess IL17F's involvement in cancer development and its potential as a therapeutic target? 03/05/2022

Tumor xenografts, immune cell infiltration analysis, and therapeutic antibody testing assess IL17F's role in cancer.

What methodologies are employed to explore IL17F's role in promoting neutrophil recruitment and activation at mucosal sites? 04/05/2021

Neutrophil chemotaxis assays, in vivo neutrophil tracking, and immune cell activation analysis explore IL17F's impact on neutrophil functions.

How do researchers differentiate the functional roles of IL17F from other IL17 family members in immune responses? 02/04/2021

Genetic knockout models, functional assays, and gene expression analyses distinguish IL17F's unique roles.

How is the interplay between IL17F and gut microbiota investigated, and what are the implications for intestinal health? 07/21/2020

Microbiota composition analysis, germ-free models, and immune cell profiling investigate IL17F-microbiota interactions.

Could you elaborate on the strategies employed to investigate IL17F's contribution to autoimmune diseases using experimental models and patient samples? 05/27/2020

Animal disease models, patient samples, and immunological assays elucidate IL17F's contribution to autoimmune diseases.

What experimental techniques are utilized to dissect the complex heterodimerization of IL17F with IL17A and its impact on signaling pathways? 04/09/2020

Co-immunoprecipitation, fluorescence resonance energy transfer (FRET), and pathway-specific assays elucidate IL17F's heterodimerization and signaling.

How do researchers investigate the potential synergistic or antagonistic effects of IL17F on tissue inflammation in comparison to other cytokines? 12/21/2019

Comparative cytokine profiling, inflammatory assays, and pathway analysis dissect IL17F's interplay with other immune mediators.

What molecular approaches are used to study IL17F's involvement in chronic inflammatory conditions, such as asthma and inflammatory bowel diseases? 03/31/2018

Allergen challenges, inflammatory disease models, and gene expression profiling probe IL17F's involvement in chronic conditions.

Can you explain the experimental designs used to study the impact of IL17F on barrier function in epithelial tissues? 06/24/2017

Epithelial barrier permeability assays, tissue section analysis, and cell culture models study IL17F's effects on barrier integrity.

How do researchers explore the effects of IL17F modulation on tissue repair and regeneration in experimental models? 03/21/2017

Wound healing assays, tissue regeneration models, and histological analyses examine IL17F's effects on tissue repair.

Customer Reviews (3)

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Reviews
11/01/2022

    High specificity.

    02/23/2020

      Good for receptor studies.

      02/05/2018

        Potent in anti-bacterial assays.

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