Active Recombinant Human LILRB3 Protein, His-Avi-tagged, Biotinylated

• Cat.No.: LILRB3-522HB
• Product Overview: Biotinylated Recombinant Human LILRB3 Protein(O75022-1)(Gly24-Glu443) is expressed from HEK293 with His tag and Avi tag at the C-terminal.

Specification

• Source: HEK293
• Species: Human
• Tag: His-Avi
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human LILRB3, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-LILRB3 Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 50 kDa. Due to glycosylation, the protein migrates to 60-72 kDa based on Tris-Bis PAGE result.
• Protein length: Gly24-Glu443
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
  > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt.
  -20 to -80°C for 3-6 months in unopened state after reconstitution.
  2-8°C for 2-7 days after reconstitution.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: LILRB3 leukocyte immunoglobulin like receptor B3 [ Homo sapiens (human) ]
• Official Symbol: LILRB3
• Synonyms: HL9; ILT5; LIR3; PIRB; CD85A; ILT-5; LIR-3; PIR-B; LILRA6; CD85 antigen-like family member A; immunoglobulin-like transcript 5; leucocyte Ig-like receptor B3; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 6; leukocyte; immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 3; monocyte inhibitory receptor HL9; leukocyte immunoglobulin-like receptor subfamily B member 3
• Gene ID: 11025
• mRNA Refseq: NM_001081450
• Protein Refseq: NP_001074919