Active Recombinant Human LTA Protein (Leu1-Leu171), N-His tagged, Animal-free, Carrier-free

• Cat.No.: LTA-214H
• Product Overview: Recombinant Human LTA Protein (Leu1-Leu171) with N-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Human TNF beta (Lymphotoxin-alpha) is a 22 kDa cytokine with 205 amino acid residues. TNF beta belongs to tumor necrosis factor (TNF) superfamily as TNF alpha. Binding to lymphotoxin-β receptor (LtβR), canonical and non-canonical NF-κB signal pathway will be activated. TNF beta is related to apoptosis and inflammatory signals
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED50 for this effect is < 3 pg/mL. The specific activity of recombinant human TNF beta is > 3.3 × 10⁸ IU/mg.
• Molecular Mass: The protein has a calculated MW of 18.65 kDa.
  The protein migrates as 21 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Leu1-Leu171
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution containing 20 mM sodium citrate and 0.2 M NaCl, pH 3.5.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: LTA lymphotoxin alpha (TNF superfamily, member 1) [ Homo sapiens (human) ]
• Official Symbol: LTA
• Synonyms: LTA; lymphotoxin alpha (TNF superfamily, member 1); TNFB; lymphotoxin-alpha; LT; TNFSF1; LT-alpha; TNF-beta; tumor necrosis factor beta; tumor necrosis factor ligand superfamily member 1
• Gene ID: 4049
• mRNA Refseq: NM_000595
• Protein Refseq: NP_000586
• MIM: 153440
• UniProt ID: P01374