Active Recombinant Human MAP3K14, GST-tagged

• Cat.No.: MAP3K14-1065H
• Product Overview: Recombinant human NIK (325-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 325-end
• Description: This gene encodes mitogen-activated protein kinase kinase kinase 14, which is a serine/threonine protein-kinase. This kinase binds to TRAF2 and stimulates NF-kappaB activity. It shares sequence similarity with several other MAPKK kinases. It participates in an NF-kappaB-inducing signalling cascade common to receptors of the tumour-necrosis/nerve-growth factor (TNF/NGF) family and to the interleukin-1 type-I receptor.
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 8 nmol/min/mg
• Molecular Mass: ~108 kDa
• Purity: >85%
• Applications: Kinase Assay; Western Blot
• Stability: 1 yr at -70oC from date of shipment.
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 ug/ul

Gene Information

• Gene Name: MAP3K14 mitogen-activated protein kinase kinase kinase 14 [ Homo sapiens ]
• Official Symbol: MAP3K14
• Synonyms: mitogen-activated protein kinase kinase kinase 14; HS; NIK; HSNIK; FTDCR1B; MAP3K14; NF-kappa-beta-inducing kinase; serine/threonine protein-kinase; serine/threonine-protein kinase NIK; EC 2.7.11.25; Serine/threonine-protein kinase NIK
• Gene ID: 9020
• mRNA Refseq: NM_003954
• Protein Refseq: NP_003945
• MIM: 604655
• UniProt ID: Q99558
• Chromosome Location: 17q21
• Pathway: Apoptosis; Epithelial cell signaling in Helicobacter pylori infection; Intestinal immune network for IgA production; MAPK signaling pathway; T cell receptor signaling pathway
• Function: MAP kinase kinase kinase activity; ATP binding; protein binding; protein kinase activity