Active Recombinant Human Matrix Metallopeptidase 2 (Gelatinase A, 72 KDa Gelatinase, 72 KDa Type IV Collagenase)

• Cat.No.: MMP2-1715H
• Product Overview: Recombinant human MMP2 was expressed in E. coli and purified by SDS PAGE and HPLC, 62.0 KDa.

Specification

• Species: Human
• Source: Human
• Tag: Non
• Description: Matrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing catalytic activity. These enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovascular disease, skeletal growth-plate disorders, and cancer metastasis. MMP-2 is a secreted collagenase with specificity toward Type IV, V, VII, and X collagens.
• Form: Supplied as a lyophilized powder.
• Purity: > 90% pure by SDS PAGE and HPLC
• Sequence: Region of MMP2 protein corresponding to amino acids MYNFFPRKPK WDKNQITYRI IGYTPDLDPE TVDDAFARAF QVWSDVTPLR FSRIHDGEAD IMINFGRWEH GDGYPFDGKD GLLAHAFAPG TGVGGDSHFD DDELWTLGEG QVVRVKYGNA DGEYCKFPFL FNGKEYNSCT DTGRSDGFLW CSTTYNFEKD GKYGFCPHEA LFTMGGNAEG QPCKF
• Molecular Mass: 62.0 kDa
• Applications: Numerous applications are possible with this product and should be tested by the end user under their own laboratory conditions
• Endotoxin Levels: Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
• Bioactivity: MMP-2 activity was measured by its ability to cleave a chromogenic peptide MMP-2 substrate at room temperature. At an MMP-2 concentration of 2.5 ug/ml, 50% cleavage was achieved at an incubation time of approximately 25 minutes.
• Storage: Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: MMP2 matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) [ Homo sapiens ]
• Official Symbol: MMP2
• Synonyms: MMP2; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); MONA; CLG4A; TBE-1; MMP-II; matrix metalloproteinase 2; collagenase type IV-A; neutrophil gelatinase; matrix metalloproteinase-II; CLG4; EC 3.4.24.24; Matrix metalloproteinase-2; MMP-2; Gelatinase A
• Gene ID: 4313
• mRNA Refseq: NM_001127891
• Protein Refseq: NP_001121363
• MIM: 120360
• UniProt ID: P08253
• Chromosome Location: 16q13-q21
• Pathway: ATF-2 transcription factor network; Angiopoietin receptor Tie2-mediated signaling; Bladder cancer; Diabetes pathways; GnRH signaling pathway; IL-3 Signaling Pathway; LPA receptor mediated events; Leukocyte transendothelial migration; Matrix Metalloproteinases; Osteopontin-mediated events; Pathways in cancer; Plasma membrane estrogen receptor signaling
• Function: metal ion binding; metalloendopeptidase activity; peptidase activity; zinc ion binding

Citation

A new generation of topical chronic wound treatments containing specific MMP inhibitors

Journal: Chronic Wound Care Management and Research    Data: 2014/9/1

Authors: Ravi Shrivastava, Claire Janicot, Pedro Neto

Article Snippet:Selection of MMPs involved in eCM destruction Based on literature review, all commercially available purified MMPs were tested.Selection of MMPs involved in eCM destruction Based on literature review, all commercially available purified MMPs were tested.. Purified recombinant human matrix MMP-1 (intestinal collagenase), MMP-2 (gelatinase), MMP-3 (stromelysin 1, progelatinase), MMP-7 (matrilysin, uterine), MMP-8 (neutrophil collagenase), MMP-9 (gelatinase B), MMP-10 (stromelysin-2), MMP-11 (stromelysin-3), MMP12 (macrophage elastase), MMP-13 (collagenase-3), MMP14 (membrane-inserted/MT1-MMP), MMP-15 (MTMMP2/ SMCP-2), MMP-16 (MMP-X2), MMP-17 (MT4-MMP), MMP-19 (MMP-RASI), MMP-20 (enamelysin), MMP-21, MMP-23 (CA-MMP), MMP-24 (MT5-MMP), MMP-26 (endometase/matrilysin), MMP-27 (MMpeptidase 27), and MMP-28 (epilysin) were purchased from Creative-Biomart (Shirley, NY, USA).. All experiments were conducted using a fixed MMP concentration of 10 μg MMP Eq/mL (equivalents of solutes per milliliter), which was selected on the basis of the median MMP concentration in chronic wound fluids.25 MMPs were then associated with each other to identify the group of epithelial or fibroblast cell matrix-destroying MMPs (VB-MMPs).All experiments were conducted using a fixed MMP concentration of 10 μg MMP Eq/mL (equivalents of solutes per milliliter), which was selected on..