Active Recombinant Human MGAT5 protein, His-tagged

• Cat.No.: MGAT5-1120H
• Product Overview: Active Recombinant Human MGAT5 protein(NP_002401.1)(Leu 189-Leu 741) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus and a signal peptide at the terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 189-741 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to transfer NAcetylαDglucosamine from UDPNAcetylαDglucosamine to a biantennary Nlinked core pentasaccharide in a CD39L3 coupled assay. The specific activity is >10pmoles/min/μg.
• Molecular Mass: The recombinant human MGAT5 consists of 564 amino acids and predictes a molecular mass of 65 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh MGAT5 is approximately 60-65 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MGAT5 mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase [ Homo sapiens ]
• Official Symbol: MGAT5
• Synonyms: MGAT5; mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase; alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A; GNT V; glcNAc-T V; N-acetylglucosaminyl-transferase V; mannoside acetylglucosaminyltransferase 5; alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase; GNT-V; GNT-VA; FLJ31899; FLJ43217; DKFZp686B24166
• Gene ID: 4249
• mRNA Refseq: NM_002410
• Protein Refseq: NP_002401
• MIM: 601774
• UniProt ID: Q09328