Active Recombinant Human MGAT5 protein, His-tagged

• Cat.No.: MGAT5-1120H
• Product Overview: Recombinant Human MGAT5 protein(NP_002401.1)(Leu189-Leu741), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to transfer N-Acetyl-α-D-glucosamine from UDP-N-Acetyl-α-D-glucosamine to a biantennary N-linked core pentasaccharide in a CD39L3 coupled assay. The specific activity is >10pmoles/min/μg
• Molecular Mass: The recombinant human MGAT5 consists of 564 amino acids and predictes a molecular mass of 65 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh MGAT5 is approximately 60-65 kDa.
• Protein length: Leu189-Leu741
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MGAT5 mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase [ Homo sapiens ]
• Official Symbol: MGAT5
• Synonyms: MGAT5; mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase; alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A; GNT V; glcNAc-T V; N-acetylglucosaminyl-transferase V; mannoside acetylglucosaminyltransferase 5; alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase; GNT-V; GNT-VA; FLJ31899; FLJ43217; DKFZp686B24166
• Gene ID: 4249
• mRNA Refseq: NM_002410
• Protein Refseq: NP_002401
• MIM: 601774
• UniProt ID: Q09328

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Q&As

What role does MGAT5 play in immune responses?

MGAT5 modulates immune responses.

What is the primary role of MGAT5 in glycoprotein synthesis?

MGAT5 is key for glycoprotein glycosylation.

How does MGAT5 contribute to cell signaling and communication?

It affects cell signaling by altering glycosylation patterns.

What is the impact of altered MGAT5 activity on cancer progression?

Altered MGAT5 activity is linked to cancer progression.

How do genetic variations in MGAT5 affect cellular functions?

Genetic changes in MGAT5 impact glycosylation and cell processes.

What potential therapeutic applications arise from targeting MGAT5 in diseases?

Targeting MGAT5 could treat diseases involving abnormal glycosylation.

How does MGAT5 interact with other enzymes in glycosylation pathways?

MGAT5 collaborates with other enzymes in glycosylation.