Active Recombinant Human NID1 protein, His-tagged

• Cat.No.: NID1-331H
• Product Overview: Recombinant Human NID1(Leu29-Lys1114 (Gln1113Arg)) fused with His tag at N-terminal was expressed in NS0.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 29-1114 a.a.
• Predicted N Terminal: His
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. When 4 x 10^4 cells/well are added to rhNidogen-1 coated plates (30 µg/mL with 100 µL/well), approximately 40-75% will adhere after one hour at 37 centigrade.
• Molecular Mass: Predicted Molecular Mass: 120 kDa;SDS-PAGE: 130 kDa, reducing conditions
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.

Gene Information

• Gene Name: NID1 nidogen 1 [ Homo sapiens ]
• Official Symbol: NID1
• Synonyms: NID1; nidogen 1; NID, nidogen (enactin); nidogen-1; entactin; NID-1; enactin; NID
• Gene ID: 4811
• mRNA Refseq: NM_002508
• Protein Refseq: NP_002499
• MIM: 131390
• UniProt ID: P14543
• Chromosome Location: 1q43
• Function: calcium ion binding; collagen binding; extracellular matrix binding; laminin-1 binding