Active Recombinant Human PDCD1 Protein, hFc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: PDCD1-032HAF555
• Product Overview: The Alexa Fluor 555 conjugated extracellular domain of human CD279 [PD-1] (NP_005009.2) (Leu25-Gln167) is fused to the N-terminus of the Fc region of human IgG1 was expressed in CHO cell.

Specification

• Description: This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases
• Source: CHO
• Species: Human
• Tag: Fc
• Form: Lyophilized
• Bio-activity: Measured by its binding ability in a functional ELISA.
• Molecular Mass: 46 kDa (monomer)
• AA Sequence: Leu25-Gln167
• Endotoxin: < 0.06 EU/ μg as determined by LAL test.
• Purity: > 98 % by SDS-PAGE under reducing conditions.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Stable for at least 1 year after receipt when stored at -20 centigrade. Working aliquots are stable for up to 3 months when stored at -20 centigrade.
• Concentration: 100 μg/mL
• Storage Buffer: Lyophilized from 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Protein length: Leu25-Gln167

Gene Information

• Gene Name: PDCD1 programmed cell death 1 [ Homo sapiens ]
• Official Symbol: PDCD1
• Synonyms: PDCD1; programmed cell death 1; programmed cell death protein 1; CD279; PD1; protein PD-1; PD-1; SLEB2; hPD-1; hPD-l
• Gene ID: 5133
• mRNA Refseq: NM_005018
• Protein Refseq: NP_005009
• MIM: 600244
• UniProt ID: Q15116

Reviews

06/06/2021

Timely antibody validation, a valuable asset for our laboratory.

02/12/2021

Precise protein quantification, fundamental for maintaining data accuracy.

06/20/2019

Dependable peptide synthesis, accelerates our peptide-based projects effectively.

Q&As

What are the mechanisms by which PDCD1 modulates the immune response to viral infections?

PDCD1 modulates responses to viral infections, affecting T cell function and viral clearance.

What are the mechanisms by which PDCD1 modulates the immune response to viral infections?

PDCD1 modulates responses to viral infections, affecting T cell function and viral clearance.

What role does PDCD1 play in the development of immune tolerance?

PDCD1 helps develop immune tolerance, preventing overactive immune responses.

How does PDCD1 influence the balance between immune activation and autoimmunity?

PDCD1 maintains a balance between immune response and autoimmunity, preventing self-reactivity.

How does PDCD1 contribute to the exhaustion of T cells in chronic infections and cancer?

PDCD1 contributes to T cell exhaustion in chronic diseases and cancers, reducing immune efficacy.

How does PDCD1 influence the balance between immune activation and autoimmunity?

PDCD1 maintains a balance between immune response and autoimmunity, preventing self-reactivity.

What is the impact of PDCD1 on the effectiveness of immunotherapy in treating cancers?

PDCD1's role in immunotherapy is pivotal, as blocking PD-1 enhances anti-tumor responses.

How does PDCD1 contribute to the exhaustion of T cells in chronic infections and cancer?

PDCD1 contributes to T cell exhaustion in chronic diseases and cancers, reducing immune efficacy.

How does PDCD1 (PD-1) regulate immune checkpoint pathways in T cells?

PDCD1 regulates T cell activity by inhibiting immune responses, crucial in immune checkpoints.

What is the impact of PDCD1 on the effectiveness of immunotherapy in treating cancers?

PDCD1's role in immunotherapy is pivotal, as blocking PD-1 enhances anti-tumor responses.

What role does PDCD1 play in the development of immune tolerance?

PDCD1 helps develop immune tolerance, preventing overactive immune responses.

How do alterations in PDCD1 expression or signaling affect autoimmune disease progression?

Changes in PDCD1 signaling impact autoimmune disease progression, influencing immune system activity.

How does PDCD1 (PD-1) regulate immune checkpoint pathways in T cells?

PDCD1 regulates T cell activity by inhibiting immune responses, crucial in immune checkpoints.

How do alterations in PDCD1 expression or signaling affect autoimmune disease progression?

Changes in PDCD1 signaling impact autoimmune disease progression, influencing immune system activity.