Active Recombinant Human PDE5A Protein, His-MBP-tagged

• Cat.No.: PDE5A-26H
• Product Overview: Active Recombinant Human PDE5A Protein(536-860 aa), fused with N-terminal His tag and MBP tag, is expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His&MBP
• Protein Length: 536-860 aa
• Form: PBS, pH7.4, containing 5% Trehalose.
• Bio-activity: PDE5A-1E is a splice variant of phosphodiesterase 5A (PDE5A), a key member of the PDE superfamily that hydrolyzes cyclic guanosine monophosphate (cGMP), a critical second messenger regulating smooth muscle relaxation, vascular tone and cellular signaling. Expressed prominently in vascular smooth muscle, penile tissue and platelets, PDE5A-1E retains the core catalytic domain of wild-type PDE5A, enabling it to degrade cGMP and modulate downstream physiological processes like penile erection and blood pressure regulation. As a unique isoform, it has distinct tissue expression patterns and functional characteristics compared to other PDE5A variants, making it a vital therapeutic target for erectile dysfunction and pulmonary arterial hypertension. Additionally, PDE5A-1E physically interacts with APRT, forming a protein complex with unclear functional implications.Thus a functional ELISA assay was conducted to detect the interaction of recombinant human PDE5A-1E and recombinant human APRT. Briefly, PDE5A-1E was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to APRT-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-PDE5A-1E pAb, then aspirated and washed 3 times. After incubas. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human PDE5A-1E and recombinant human APRT was shown, the EC50 for this effect is 0.128µg/mL.
• Molecular Mass: 79.6kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 90%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month, at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: PDE5A phosphodiesterase 5A, cGMP-specific [ Homo sapiens ]
• Official Symbol: PDE5A
• Synonyms: PDE5A; phosphodiesterase 5A, cGMP-specific; cGMP-specific 3,5-cyclic phosphodiesterase; phosphodiesterase isozyme 5; cGMP-specific phosphodiesterase PDE5A2; cGMP-specific phosphodiesterase type 5A; cGMP-binding cGMP-specific 3,5-cyclic nucleotide phosphodiesterase; CN5A; PDE5; CGB-PDE
• Gene ID: 8654
• mRNA Refseq: NM_001083
• Protein Refseq: NP_001074
• MIM: 603310
• UniProt ID: O76074