Active Recombinant Human TGFA Protein

• Cat.No.: TGFA-244H
• Product Overview: Recombinant Human TGFA Protein without tag was expressed in E. coli.

Specification

• Description: Tumor growth factor alpha (TGF-α) is a member of the epidermal growth factor (EGF) family. TGF-α function is mediated through binding the EGF receptor (EGFR) to activate receptor tyrosine kinase signaling. TGF-α functions as a mitogen to activate epithelial cell proliferation, growth, and differentiation. In the gastric mucosa, TGF-α production inhibits gastric acid secretion and therefore plays a central role in the pathogenesis of the stomach syndrome Ménétrier’s disease. TGF-α is also produced in adult macrophages, brain cells, keratinocytes, and is widely expressed in cancer cells.
• Source: E. coli
• Species: Human
• Bio-activity: 3T3 cell proliferation, ED50≤2 ng/mL
• Molecular Mass: Monomer, 5.6 kDa (with 50 amino acids)
• AA Sequence: VVSHFNDCPDSHTQFCFHGTCRFLV QEDKPACVCHSGYVGARCEHADLLA
• Endotoxin: ≤1 EUs/μg, Kinetic LAL (50% confidence)
• Purity: ≥95%, Reducing and Non-Reducing SDS PAGE
• Stability: 12 months from date of receipt when stored at -20 to -80 centigrade as supplied.
  1 month when stored at 4 centigrade after reconstituting as directed.
  3 months when stored at -20 to -80 centigrade after reconstituting as directed.
• Storage: Storage Prior to Reconstitution: -20 centigrade
• Storage Buffer: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
• Reconstitution: Sterile water at 0.1 mg/mL
• Shipping: Room temperature
• Instructions: Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80 centigrade and avoid repeat freeze thaws.

Gene Information

• Gene Name: TGFA transforming growth factor, alpha [ Homo sapiens (human) ]
• Official Symbol: TGFA
• Synonyms: TGFA; transforming growth factor, alpha; protransforming growth factor alpha; TGF-alpha; TFGA
• Gene ID: 7039
• mRNA Refseq: NM_001099691
• Protein Refseq: NP_001093161
• MIM: 190170
• UniProt ID: P01135

Reviews

05/03/2020

Swift and accurate service, essential for research success.

04/14/2020

Invaluable support for insights, a research cornerstone.

12/21/2018

Trustworthy results, supports research consistency.

Q&As

How does TGFA contribute to cell growth and proliferation?

It stimulates cell proliferation and survival, playing a key role in tissue development.

How do genetic variations in TGFA affect tissue repair?

Genetic mutations in TGFA can lead to impaired wound healing and tissue regeneration.

What is the impact of altered TGFA activity on cancer development?

Altered TGFA activity is linked with cancer progression, as it can stimulate uncontrolled cell growth.

What is the primary role of TGFA in cell signaling?

TGFA, a growth factor, is vital for activating signaling pathways that control cell growth and differentiation.

What potential therapeutic applications arise from targeting TGFA in regenerative medicine and oncology?

Targeting TGFA offers potential for treating wounds and cancer by modulating growth factor signaling.

How does TGFA interact with other growth factors and receptors?

TGFA interacts with epidermal growth factor receptors, influencing various cellular processes.

What role does TGFA play in wound healing?

TGFA is important in wound healing, promoting the regeneration of skin and other tissues.