Active Recombinant Human TWEAK, FLAG-tagged

• Cat.No.: TNFSF12-70H
• Product Overview: Produced in E. coli. The extracellular domain of human TWEAK (aa 106-249) is fused at the N-terminus to a linker peptide (11 aa) and a FLAG®-tag.

Specification

• Species: Human
• Source: E.coli
• Tag: Flag
• Protein Length: 106-249 a.a.
• Form: Lyophilized. Contains PBS.
• Bio-activity: Kills TWEAK sensitive cells. Has a pro-apoptotic effect on several target cell lines and induces angiogenesis and proliferation in endothelial cells. Binds to human and mouse Fn14(TWEAKR).
• Molecular Mass: ~18-20 kDa as determined by SDS-PAGE.
• Purity: >95% as determined by SDS-PAGE. Endotoxin:<0.1 eu/μg="" of="" purified="" protein="" (lal="">test).
• Usage: For in vitro research use only. Not for use in diagnostics or in humans.
• Storage: Store lyophilized protein at at -20°C. After reconstitution, aliquot and store at -20°C.
• Reconstitution: Reconstitute in 100 μL sterile water for a 0.1 mg/mL solution. Further dilutions should be made with medium containing 5% fetal calf serum.
• Warning: No warranties, expressed or implied, are made regarding the use of this product. Creative Biomart is not liable for any damage, personal injury, or economic loss caused by this product.

Gene Information

• Gene Name: TNFSF12 tumor necrosis factor (ligand) superfamily, member 12 [ Homo sapiens ]
• Official Symbol: TNFSF12
• Synonyms: TNFSF12; tumor necrosis factor (ligand) superfamily, member 12; tumor necrosis factor ligand superfamily member 12; APO3L; DR3LG; TWEAK; APO3 ligand; APO3/DR3 ligand; TNF-related WEAK inducer of apoptosis; MGC20669; MGC129581
• Gene ID: 8742
• mRNA Refseq: NM_003809
• Protein Refseq: NP_003800
• MIM: 602695
• UniProt ID: O43508
• Chromosome Location: 17p13.1
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Gene Expression, organism-specific biosystem; Regulation of mRNA Stability by Proteins that Bind AU-rich Elements, organism-specific biosystem; Stabilization of mRNA by HuR, organism-specific biosystem
• Function: cytokine activity; cytokine activity; protein binding; receptor binding; tumor necrosis factor receptor binding