Active Recombinant Human USP7 protein, His-tagged

• Cat.No.: USP7-490H
• Product Overview: Recombinant Human full length USP7(1-1102) fused with N terminal His tag was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Bio-activity: The activity of His-USP7 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysedcleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of His-USP7 was compared confirming the deubiquitylating activity of His-USP7.
• Molecular Mass: ~130 kDa
• AA Sequence: MGSSHHHHHHSSG LEVLFQGP G S M NHQQQQQQQKAGEQQLSEPEDMEME AGDTDDPPRITQNPVINGNVALSDG HNTAEEDMEDDTSWRSEATFQFTVE RFSRLSESVLSPPCFVRNLPWKIMV MPRFYPDRPHQKSVGFFLQCNAESD STSWSCHAQAVLKIINYRDDEKSFS RRISHLFFHKENDWGFSNFMAWSEV TDPEKGFIDDDKVTFEVFVQADAPH GVAWDSKKHTGYVGLKNQGATCYMN SLLQTLFFTNQLRKAVYMMPTEGDD SSKSVPLALQRVFYELQHSDKPVGT KKLTKSFGWETLDSFMQHDVQELCR VLLDNVENKMKGTCVEGTIPKLFRG KMVSYIQCKEVDYRSDRREDYYDIQ LSIKGKKNIFESFVDYVAVEQLDGD NKYDAGEHGLQEAEKGVKFLTLPPV LHLQLMRFMYDPQTDQNIKINDRFE FPEQLPLDEFLQKTDPKDPANYILH AVLVHSGDNHGGHYVVYLNPKGDGK WCKFDDDVVSRCTKEEAIEHNYGGH DDDLSVRHCTNAYMLVYIRESKLSE VLQAVTDHDIPQQLVERLQEEKRIE AQKRKERQEAHLYMQVQIVAEDQFC GHQGNDMYDEEKVKYTVFKVLKNSS LAEFVQSLSQTMGFPQDQIRLWPMQ ARSNGTKRPAMLDNEADGNKTMIEL SDNENPWTIFLETVDPELAASGATL PKFDKDHDVMLFLKMYDPKTRSLNY CGHIYTPISCKIRDLLPVMCDRAGF IQDTSLILYEEVKPNLTERIQDYDV SLDKALDELMDGDIIVFQKDDPEND NSELPTAKEYFRDLYHRVDVIFCDK TIPNDPGFVVTLSNRMNYFQVAKTV AQRLNTDPMLLQFFKSQGYRDGPGN PLRHNYEGTLRDLLQFFKPRQPKKL YYQQLKMKITDFENRRSFKCIWLNS QFREEEITLYPDKHGCVRDLLEECK KAVELGEKASGKLRLLEIVSYKIIG VHQEDELLECLSPATSRTFRIEEIP LDQVDIDKENEMLVTVAHFHKEVFG TFGIPFLLRIHQGEHFREVMKRIQS LLDIQEKEFEKFKFAIVMTGRHQYI NEDEYEVNLKDFEPQPGNMSHPRPW LGLDHFNKAPKRSRYTYLEKAIKIH N
• Purity: >80% by SDS-PAGE
• Stability: 12 months at -70 centigrade; aliquot as required
• Storage: Storage at -70 centigrade
• Concentration: 0.5 mg/ml

Gene Information

• Gene Name: USP7 ubiquitin specific peptidase 7 (herpes virus-associated) [ Homo sapiens ]
• Official Symbol: USP7
• Synonyms: USP7; ubiquitin specific peptidase 7 (herpes virus-associated); HAUSP, ubiquitin specific protease 7 (herpes virus associated); ubiquitin carboxyl-terminal hydrolase 7
• Gene ID: 7874
• mRNA Refseq: NM_003470
• Protein Refseq: NP_003461
• MIM: 602519
• UniProt ID: Q93009
• Chromosome Location: 16p13.3
• Pathway: FoxO family signaling, organism-specific biosystem; Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem; p53 pathway, organism-specific biosystem
• Function: cysteine-type endopeptidase activity; p53 binding; peptidase activity; protein C-terminus binding; protein binding