Active Recombinant Mouse Alpi Protein, His-tagged

• Cat.No.: Alpi-10998M
• Product Overview: Mouse ALPI Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Pro 503 (Accession # Q0VD75). This protein carries a polyhistidine tag at the C-terminus. Predicted N-terminus: Ile 20. The protein is designed as a dimer.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 20-503 aa
• Description: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is a component of the gut mucosal defense system and is thought to function in the detoxification of lipopolysaccharide, and in the prevention of bacterial translocation in the gut.
• Form: Supplied as 0.2 μm filtered solution in 20 mM Tris, 150 mM NaCl, 20% Glycerol, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Mouse ALPI Protein, His Tag at 5 μg/mL (100 μL/well) can bind various dilution ratio of Anti-ALPP/ALPG/ALPI Polyclonal Antibody.
• Molecular Mass: The protein has a calculated MW of 54.2 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU/μg by the LAL method/rFC method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: This product is stable after storage at: The product MUST be stored at -70 centigrade or lower upon receipt; -70 centigrade for 3 months under sterile conditions. Please avoid repeated freeze-thaw cycles.
• Concentration: 0.2 mg/mL
• Shipping: This product is supplied and shipped with dry ice, please inquire the shipping cost.

Gene Information

• Gene Name: Alpi alkaline phosphatase, intestinal [ Mus musculus ]
• Official Symbol: ALPI
• Synonyms: ALPI; alkaline phosphatase, intestinal; intestinal alkaline phosphatase; alkaline phosphatase 6; Akp6; gIAP; 2010001C14Rik
• Gene ID: 76768
• mRNA Refseq: NM_001081082
• Protein Refseq: NP_001074551
• UniProt ID: Q0VD75