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Active Recombinant Mouse Car14 Protein, His-tagged

Cat.No. : Car14-822M
Product Overview : Recombinant extracellular domain of mouse Car14 (NP_035927.1) (Met 1-Met 290) was expressed with a C-terminal polyhistidine tag.
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Source : HEK293
Species : Mouse
Tag : His
Predicted N Terminal : Ala 16
Form : Lyophilized from sterile 25mM Tris, 0.15mM NaCl, pH 7.51, 5%~8% trehalose and mannitol.
Bio-activity : Measured by its esterase activity. The specific activity is >400 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 0.8 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
Molecular Mass : The recombinant mouse CA14 consists of 286 amino acids and has a calculated molecular mass of 32.2 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 45-48 kDa protein in SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >95 % as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Gene Name : Car14 carbonic anhydrase 14 [ Mus musculus ]
Official Symbol : Car14
Synonyms : Ca14; AW536446; CA XIV; CA-XIV; carbonate dehydratase XIV; carbonic anhydrase XIV
Gene ID : 23831
mRNA Refseq : NM_011797
Protein Refseq : NP_035927
MIM :
UniProt ID : Q9WVT6
Chromosome Location : 3; 3 F2.1
Pathway : Metabolism, organism-specific biosystem; Nitrogen metabolism, organism-specific biosystem; Nitrogen metabolism, conserved biosystem
Function : carbonate dehydratase activity; lyase activity; metal ion binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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Are there any known small molecule modulators specific to CA14's activity? 10/16/2022

Specific modulators can be identified through high-throughput screening assays targeting CA14 and validated using in vitro and in vivo models.

What unique functional attributes differentiate CA14 from other carbonic anhydrase isoforms? 02/14/2022

CA14's unique functional attributes can be discerned using comparative enzymatic assays and structural analyses against other isoforms.

How do changes in cellular pH or ion concentrations influence CA14 activity? 09/26/2020

Influence of pH or ions on CA14 can be analyzed using enzymatic assays under varying conditions and ion-sensitive probes.

Are there any known disease associations where CA14 mutations or dysregulation play a pivotal role? 05/02/2020

Disease associations can be determined through genetic studies, patient tissue analyses, and literature reviews focusing on CA14's relevance in pathological conditions.

In what tissues or cell types is CA14 predominantly expressed, and how does this correlate with its physiological role? 09/17/2019

Predominant tissue or cell-type expression can be determined using tissue microarrays, qPCR, or proteomic databases, correlating findings with potential physiological implications.

What are the consequences of CA14 overexpression or knockdown in cellular and organismal models? 02/16/2018

Consequences of overexpression or knockdown can be assessed using phenotypic analyses, cellular viability assays, and in vivo studies in organisms with altered CA14 expression.

Are there specific cellular pathways where CA14 plays a crucial modulatory role? 07/15/2017

CA14's role in cellular pathways can be explored using pathway enrichment analyses, proteomic studies, and pathway-specific assays in CA14-modified cells.

Customer Reviews (3)

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Reviews
08/19/2022

    Reliable source, ensures reproducibility in our research.

    11/10/2020

      Efficient services, optimize our protein-related studies.

      10/01/2020

        Responsive customer support, aids in problem-solving effectively.

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