Recombinant human CA14, His-tagged
Cat.No. : | CA14-2485H |
Product Overview : | Recombinant human CA14 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
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Description : | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA14 is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII; however, they have different patterns of tissue-specific expression and thus may play different physiologic roles. |
Source : | E.coli |
Species : | Human |
Tag : | His |
Form : | Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT |
Molecular Mass : | 33.2kDa (298aa) confirmed by MALDI-TOF |
AA Sequence : | MGSSHHHHHH SSGLVPRGSH MGSADGGQHW TYEGPHGQDH WPASYPECGN NAQSPIDIQT DSVTFDPDLP ALQPHGYDQP GTEPLDLHNN GHTVQLSLPS TLYLGGLPRK YVAAQLHLHW GQKGSPGGSE HQINSEATFA ELHIVHYDSD SYDSLSEAAE RPQGLAVLGI LIEVGETKNI AYEHILSHLH EVRHKDQKTS VPPFNLRELL PKQLGQYFRY NGSLTTPPCY QSVLWTVFYR RSQISMEQLE KLQGTLFSTE EEPSKLLVQN YRALQPLNQR MVFASFIQAG SSYTTGEM |
Purity : | >85 % by SDS - PAGE |
Applications : | SDS-PAGE |
Storage : | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
Concentration : | 0.5 mg/ml (determined by Bradford assay) |
Gene Name : | CA14 carbonic anhydrase XIV [ Homo sapiens ] |
Official Symbol : | CA14 |
Synonyms : | CA14; carbonic anhydrase XIV; carbonic anhydrase 14; CA-XIV; carbonic dehydratase; carbonate dehydratase XIV; CAXiV; |
Gene ID : | 23632 |
mRNA Refseq : | NM_012113 |
Protein Refseq : | NP_036245 |
MIM : | 604832 |
UniProt ID : | Q9ULX7 |
Chromosome Location : | 1q21 |
Function : | carbonate dehydratase activity; lyase activity; metal ion binding; |
Products Types
◆ Recombinant Protein | ||
CA14-0236H | Recombinant Human CA14 Protein, GST-Tagged | +Inquiry |
CA14-1156M | Recombinant Mouse CA14 Protein, His (Fc)-Avi-tagged | +Inquiry |
CA14-424R | Recombinant Rhesus Macaque CA14 Protein, His (Fc)-Avi-tagged | +Inquiry |
CA14-2030H | Recombinant Human CA14 Protein, MYC/DDK-tagged | +Inquiry |
Car14-1960M | Recombinant Mouse Car14 Protein, Myc/DDK-tagged | +Inquiry |
◆ Lysates | ||
CA14-2802HCL | Recombinant Human CA14 cell lysate | +Inquiry |
CA14-3064MCL | Recombinant Mouse CA14 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Write a reviewReliable source, ensures reproducibility in our research.
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Q&As (7)
Ask a questionSpecific modulators can be identified through high-throughput screening assays targeting CA14 and validated using in vitro and in vivo models.
CA14's unique functional attributes can be discerned using comparative enzymatic assays and structural analyses against other isoforms.
Influence of pH or ions on CA14 can be analyzed using enzymatic assays under varying conditions and ion-sensitive probes.
Disease associations can be determined through genetic studies, patient tissue analyses, and literature reviews focusing on CA14's relevance in pathological conditions.
Predominant tissue or cell-type expression can be determined using tissue microarrays, qPCR, or proteomic databases, correlating findings with potential physiological implications.
Consequences of overexpression or knockdown can be assessed using phenotypic analyses, cellular viability assays, and in vivo studies in organisms with altered CA14 expression.
CA14's role in cellular pathways can be explored using pathway enrichment analyses, proteomic studies, and pathway-specific assays in CA14-modified cells.
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