Active Recombinant Mouse Egf protein, hFc-tagged, Low Endotoxin

• Cat.No.: Egf-868M
• Product Overview: Recombinant Mouse Egf protein(P01132)(977-1029 aa), fused with N-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Protein Length: 977-1029 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.4 - 3 ng/mL.
• Molecular Mass: The protein has a predicted MW of 32.35 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Egf epidermal growth factor [ Mus musculus ]
• Official Symbol: Egf
• Synonyms: EGF; epidermal growth factor; pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF); AI790464
• Gene ID: 13645
• mRNA Refseq: NM_010113
• Protein Refseq: NP_034243