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Recombinant Mouse Egf protein

Cat.No. : Egf-05M
Product Overview : Recombinant Mouse Egf protein was expressed in Escherichia coli.
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  • Gene Information
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Description : Epidermal Growth Factor (EGF) was originally discovered in crude preparations of nerve growth factor prepared from mouse submaxillary glands as an activity that induced early eyelid opening, incisor eruption, hair growth inhibition, and stunting of growth when injected into newborn mice. It is prototypic of a family of growth factors that are derived from membrane-anchored precursors. All members of this family are characterized by the presence of at least one EGF structural unit (defined by the presence of a conserved 6 cysteine motif that forms three disulfide bonds) in their extracellular domain. EGF is initially synthesized as a 130 kDa precursor transmembrane protein containing 9 EGF units. The mature soluble EGF sequence corresponds to the EGF unit located proximal to the transmembrane domain. The membrane EGF precursor is capable of binding to the EGF receptor and was reported to be biologically active. Mature mouse EGF shares 70 % a.a. sequence identity with mature human EGF.
Source : E.coli
Species : Mouse
Form : Lyophilized from a 0.2μm filtered solution in PBS, pH 7.4.
Bio-activity : Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁷ IU/mg.
Molecular Mass : Approximately 6.0 kDa, a single non-glycosylated polypeptide chain containing 53 amino acids, including 3 intramolecular disulfide-bonds.
Protein length : 53
AA Sequence : NSYPGCPSSYDGYCLNGGVCMHIES LDSYTCNCVIGYSGDRCQTRDLRWW ELR
Endotoxin : Less than 1 EU/μg of rMuEGF as determined by LAL method.
Purity : >97% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name : Egf
Official Symbol : Egf
Synonyms : EGF; epidermal growth factor; pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF); AI790464;
Gene ID : 13645
mRNA Refseq : NM_010113
Protein Refseq : NP_034243
UniProt ID : P01132

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How is EGF protein produced in the body? 02/07/2022

The body naturally produces EGF protein, and it can also be synthesized for clinical applications using biotechnological methods.

Are there any side effects associated with the clinical use of EGF protein? 12/06/2021

Generally, EGF is well-tolerated, but localized irritation or redness at the application site can occur in some cases.

Can EGF be used in the treatment of burns? 11/03/2021

Yes, EGF has been explored for its potential in treating burns by promoting the regeneration of damaged skin tissue.

What are the primary clinical applications of EGF protein? 10/19/2021

EGF protein is widely used in clinical settings for wound healing, tissue regeneration, and certain dermatological conditions.

Is EGF used in cosmetic products, and if so, for what purpose? 09/28/2019

Yes, EGF is a common ingredient in some cosmetic products due to its ability to promote skin rejuvenation and reduce the appearance of fine lines and wrinkles.

Customer Reviews (3)

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Reviews
01/27/2020

    This can be advantageous in understanding disease mechanisms and developing novel therapeutics.

    04/12/2018

      The manufacturer can provide detailed documentation on the quality control measures undertaken during protein production and purification.

      05/09/2016

        The manufacturer can offer technical expertise and guidance to researchers regarding the proper use, handling, and storage of EGF protein.

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