Active Recombinant Mouse FGF12 Protein

Cat.No. : FGF12-33M
Product Overview : Recombinant Mouse FGF12 Protein (Met1-Thr181) is produced by E. coli expression system.
  • Specification
  • Gene Information
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Source : E. coli
Species : Mouse
Form : 0.22 µm filtered protein solution is in 20 mM MOPS, 150 mM Na2SO4, 1 mM EDTA, 10 mM DTT, pH 7.5.
Bio-activity : The activity was measured by the binding to human FGFR4-Fc using functional ELISA. The ED50 is 0.01-0.05 µg/ml when human FGFR4-Fc is immobilized at 5 µg/ml (100 µl/ well)
Molecular Mass : The 181 amino acid recombinant protein has a predicted molecular mass of approximately 21 kD. The DTT-reduced protein migrates at approximately 21 by SDS-PAGE. The predicted N-terminal amino acid is Met.
Protein length : Met1-Thr181
Endotoxin : < 0.1 EU/µg cytokine as determined by the LAL method.
Purity : > 98%, as determined by Coomassie stained SDS-PAGE.
Applications : Bioassay
Storage : Unopened vial can be stored between 2 centigrade and 8 centigrade for one month, at -20 centigrade for six months, or at -70 centigrade for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 centigrade to -70 centigrade. Stock solutions can also be prepared at 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA and stored in working aliquots at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw cycles.
Concentration : 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial.
Gene Name : Fgf12 fibroblast growth factor 12 [ Mus musculus ]
Official Symbol : FGF12
Synonyms : FGF12; fibroblast growth factor 12; FHF-1; FGF-12; Fhf1; AV114868;
Gene ID : 14167
mRNA Refseq : NM_010199
Protein Refseq : NP_034329

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.


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