Active Recombinant Mouse Hc protein, His&hFc-tagged

Cat.No. : Hc-3716M
Product Overview : Active Recombinant Mouse Hc protein(P06684)(679~755 aa), fused with N-terminal His tag and C-terminal hFc tag, was expressed in HEK293.
  • Specification
  • Gene Information
  • Related Products
  • Download
Species : Mouse
Source : HEK293
Tag : Fc&His
Protein Length : 679-755 aa
Form : Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Bio-activity : Complement Component 5a (C5a) is a component of the complement system which plays a key role in promoting migration and adherence of neutrophils and monocytes to vessel walls. The activity of recombinant human C5a was also measured by its ability to induce N-acetyl-β-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. 3.2*10^6 differentiated U937 cells were added to the 24-well plate and different concentrations of rhC5a was added to the 24-well plate and incubated at 37°C for 3min. The cells were centrifuged at 400 g for 3min, and the supernatant contained N-acetyl-β-D-glucosaminidase. The enzyme activity of N-acetyl-β-D-glucosaminidase was measured by the substrate of 4-Nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside. It was obvious that rhC5a can significantly induce N-acetyl-β-D-glucosaminidase release.
Molecular Mass : 39.9kDa
Endotoxin : <1.0EU per 1μg (determined by the LAL method).
Purity : > 90% as determined by SDS-PAGE.
Storage : Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
Reconstitution : Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name Hc hemolytic complement [ Mus musculus ]
Official Symbol Hc
Synonyms HC; hemolytic complement; complement C5; C5; He; C5a;
Gene ID 15139
mRNA Refseq NM_010406
Protein Refseq NP_034536

Not For Human Consumption!

Inquiry

  • Reviews (0)
  • Q&As (0)

Customer Reviews

Write a review

Ask a Question for All Hc Products

Required fields are marked with *

My Review for All Hc Products

Required fields are marked with *

0
cart-icon
0
compare icon