| Species : | Mouse | 
                                
                                    | Source : | HEK293 | 
                                
                                    | Tag : | Fc&His | 
                                
                                    | Protein Length : | 679-755 aa | 
                                
                                    | Form : | Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. | 
                                
                                    | Bio-activity : | Complement Component 5a (C5a) is a component of the complement system which plays a key role in promoting migration and adherence of neutrophils and monocytes to vessel walls. The activity of recombinant human C5a was also measured by its ability to induce N-acetyl-β-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. 3.2*10^6 differentiated U937 cells were added to the 24-well plate and different concentrations of rhC5a was added to the 24-well plate and incubated at 37°C for 3min. The cells were centrifuged at 400 g for 3min, and the supernatant contained N-acetyl-β-D-glucosaminidase. The enzyme activity of N-acetyl-β-D-glucosaminidase was measured by the substrate of 4-Nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside. It was obvious that rhC5a can significantly induce N-acetyl-β-D-glucosaminidase release. | 
                                
                                    | Molecular Mass : | 39.9kDa | 
                                
                                    | Endotoxin : | <1.0EU per 1μg (determined by the LAL method). | 
                                
                                    | Purity : | > 90% as determined by SDS-PAGE. | 
                                
                                    | Storage : | Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. | 
                                
                                    | Reconstitution : | Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |