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Active Recombinant Mouse Il18 Protein (Asn36-Ser192), C-His tagged, Animal-free, Carrier-free

Cat.No. : Il18-53M
Product Overview : Recombinant Mouse Il18 Protein (Asn36-Ser192) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.
  • Specification
  • Gene Information
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Description : Interleukin-18 (IL-18) belongs to the IL-1 superfamily and is constitutively produced by macrophages, dendritic cells (DCs) and other cells. IL-18 binds to the receptor of IL-18 (IL-18R) and initiate the recruitment and heterodimerization of the IL-18RAcP, leading to downstream activation of NF-κB. After stimulation with IL-18, multiple cytokines including IFN-γ, IL-13, IL-4, IL-8, and GM-CSF and both Th1 and Th2 lymphokines could be produced by different cells. As an immunoregulaory cytokine, IL-18 can promote development of T helper 1 (Th1) cells, which maximizes the production of IFN by synergistically stimulating to mature Th1 effectors in combination with IL-12. On the contrary, it inhibits the production of the anti-inflammatory cytokine IL-10. In addition, IL-18 exhibits multiple proinflammatory functions, such as increases in cell adhesion molecules, nitric oxide synthesis, and chemokine production.
Source : E. coli
Species : Mouse
Tag : C-His
Form : Lyophilized
Bio-activity : Measure by its ability to induce IFN gamma secretion in KG-1 cells. The ED50 for this effect is < 0.5 μg/mL.
Molecular Mass : The protein has a calculated MW of 19.1 kDa.
The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).
Protein Length : Asn36-Ser192
Endotoxin : < 0.1 EU/μg of the protein by the LAL method.
Purity : >98% as determined by SDS-PAGE analysis.
Storage : This product is stable after storage at:
-20 centigrade for 12 months in lyophilized state from date of receipt.
-20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
Storage Buffer : Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
Reconstitution : It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping : The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.
Gene Name : Il18 interleukin 18 [ Mus musculus (house mouse) ]
Official Symbol : Il18
Synonyms : IL18; interleukin 18; interleukin-18; IL-1 gamma; interleukin-1 gamma; IFN-gamma-inducing factor; interferon gamma-inducing factor; interferon-gamma-inducing factor; Igif; Il-18;
Gene ID : 16173
mRNA Refseq : NM_008360
Protein Refseq : NP_032386
UniProt ID : P70380

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.



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