Active Recombinant Rhesus Macaque IL18

• Cat.No.: IL18-370R
• Product Overview: Recombinant Rhesus Macaque IL18 (Accession # AAK13416) Tyr37-Asp193 was produced in E. coli-derived.

Specification

• Species: Rhesus macaque
• Source: E.coli
• Tag: Non
• Protein Length: 37-193 a.a.
• Predicted N Terminal: Tyr37
• Form: Lyophilized from a 0.2 µm filtered solution in MOPS, Na2SO4, EDTA and DTT with BSA as a carrier protein.
• Bio-activity: Measured by its ability to induce IFN-gamma secretion by KG-1 human acute myelogenous leukemia cells in the presence of TNF-alpha.The ED50 for this effect is typically 1-4 ng/mL in the presence of 20 ng/mL recombinant human TNF-alpha.
• Molecular Mass: Recombinant Rhesus Macaque IL18 has a calculated MW of 18.2 kDa.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 10 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: IL18 interleukin 18 (interferon-gamma-inducing factor) [ Macaca mulatta (Rhesus monkey) ]
• Official Symbol: IL18
• Synonyms: IL18; interleukin 18 (interferon-gamma-inducing factor); interleukin-18; interleukin-18 proprotein
• Gene ID: 574151
• mRNA Refseq: NM_001032834
• Protein Refseq: NP_001028006
• Chromosome Location: 14

Q&As

What are the difference between IL18-01HG, IL18-153HG and IL18-500H. (sequence, biological activity, QC, or what do you propose to buy)

IL18-01HG: Sequence: a.a.37-193 Endotoxin: <20 EU/mg Bio-activity: >5.0 x 10^4 IU/mg QC: Activity test, SDS-PAGE, LAL, SEC-HPLC IL18-153HG: Protein length: Tyr37-Asp193 AA Sequence: YFGKLESKLS VIRNLNDQVL FIDQGNRPLF EDMTDSDCRD NAPRTIFIISMYKDSQPRGM AVTISVKCEK ISTLSCENKI ISFKEMNPPD NIKDTKSDIIFFQRSVPGHD NKMQFESSSY EGYFLACEKE RDLFKLILKK EDELGDRSIMFTVQNEDVD Bio-activity: 1.0*10^6IU Measured by its ability to induce IFN-gamma secretion by KG‐1 human acute myelogenous leukemia cells in the presence of TNF-alpha. QC: Activity test, SDS-PAGE, LAL IL18-500H Recombinant Human IL18 will be custom produced upon order. All the sequence, purity, activity, and QC procedures will be optional and decided by our customer.