Active Recombinant Mouse Il1f5 Protein

• Cat.No.: Il1f5-148M
• Product Overview: Purified recombinant protein of Mouse interleukin 1 family, member 5 (delta) (Il1f5), transcript variant 2 without tag was expressed in E.coli.

Specification

• Description: Inhibits the activity of interleukin-36 (IL36A,IL36B and IL36G) by binding to receptor IL1RL2/IL-36R and preventing its association with the coreceptor IL1RAP for signaling. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor. Proposed to play a role in skin inflammation. May be involved in the innate immune response to fungal pathogens. May activate an anti-inflammatory signaling pathway by recruiting SIGIRR.
• Source: E. coli
• Species: Mouse
• Bio-activity: Measured by its ability to inhibit IL-36γ induced IL-6 production by human PBMCs.
• Molecular Mass: 17 kDa
• AA Sequence: VLSGALCFRMKDSALKVLYLHNNQL LAGGLHAEKVIKGEEISVVPNRALD ASLSPVILGVQGGSQCLSCGTEKGP ILKLEPVNIMELYLGAKESKSFTFY RRDMGLTSSFESAAYPGWFLCTSPE ADQPVRLTQIPEDPAWDAPITDFYF QQCD
• Endotoxin: Endotoxin level is < 0.1 ng/µg of protein (< 1 EU/µg)
• Purity: >95%, as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for at least 6 months from date of receipt under proper storage and handling conditions.
• Storage: Store at -80 centigrade.
• Storage Buffer: Lyophilized from a 0.2 µM filtered solution of 20mM phosphate buffer,100mM NaCl, pH 7.2
• Reconstitution: Resuspend the protein in the desired concentration in proper buffer.

Gene Information

• Gene Name: Il1f5 interleukin 1 family, member 5 (delta) [ Mus musculus (house mouse) ]
• Official Symbol: Il1f5
• Synonyms: Il1f5; interleukin 1 family, member 5 (delta); IL36RN; Il-1h3; Il1hy1; IL-36Ra; AI413231; Fil1delta; interleukin-36 receptor antagonist protein; IL-1 delta; IL-1F5; IL-1HY1; IL-1L1; interleukin 1 receptor antagonist homolog 1; interleukin-1 HY1; interleukin-1 delta; interleukin-1 family member 5; interleukin-1 homolog 3; interleukin-1-like protein 1
• Gene ID: 54450
• mRNA Refseq: NM_019451
• Protein Refseq: NP_062324
• UniProt ID: Q9QYY1

Reviews

05/31/2021

When conducting experiments with IL36RN, clear and consistent results can be obtained, facilitating data analysis and interpretation.

01/29/2019

IL36RN has high specific activity and can exert strong biological effect at low concentration.

01/11/2019

The stability of the IL36RN product allows it to cope with different experimental conditions and modes of operation.

Q&As

What is the action mechanism of IL36RN?

The mechanism of action of IL36RN is to play an anti-inflammatory, anti-infection, anti-tumor and other biological mechanisms by acting on the signaling pathway of IL-36 receptor.

How to ensure the purity of IL36RN when preparing it?

The purity of IL36RN can be ensured by ion exchange chromatography, dialysis, gel filtration and other methods.

IL36RN production process is what?

Mass production of IL36RN can be carried out through large-scale fermentation, purification and other processes.

What is the difference between IL36RN protein and IL36α, β, γ?

This protein is an inhibitor with no biological activity of its own; Recombinant IL36α, β and γ are agonists with different biological effects.

Can recombinant IL36RN be used in clinical trials and therapeutics?

Can be used in clinical trials and therapy.

Does IL36RN have sustained and stable biological activity?

IL36RN has sustained and stable biological activity.