Recombinant Mouse Il1f5 protein

• Cat.No.: Il1f5-553M
• Product Overview: Recombinant Mouse Il1f5 protein was expressed in Escherichia coli.

Specification

• Description: Interleukin-36 receptor antagonist (IL-36RA) is a secreted protein which belongs to the interleukin 1 cytokine family (IL-1 family) and it is predominantly expressed in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. IL-36RA has been reported to antagonize the biological activity of IL-36α (IL-1F6), IL-36β (IL-1F8), and IL-36γ (IL-1F9). Furthermore, it could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1). In addition, The receptor for IL-36RA has not been positively identified. Indirect evidence suggests it is IL-1Rrp2. Recombinant murine interleukin-36 RA contains 154 amino acid residues and it shares 91 % a.a. sequence identity with human IL-36RA.
• Source: E.coli
• Species: Mouse
• Form: Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
• Bio-activity: Measured by its ability to inhibit IL-36γ induced IL-6 production by human PBMCs.
• Molecular Mass: Approximately 16.9 kDa, a single non-glycosylated polypeptide chain containing 154 amino acids.
• Protein length: 154
• AA Sequence: VLSGALCFRMKDSALKVLYLHNNQL LAGGLHAEKVIKGEEISVVPNRALD ASLSPVILGVQGGSQCLSCGTEKGP ILKLEPVNIMELYLGAKESKSFTFY RRDMGLTSSFESAAYPGWFLCTSPE ADQPVRLTQIPEDPAWDAPITDFYF QQCD
• Endotoxin: Less than 1 EU/µg of rMuIL-36RA as determined by LAL method.
• Purity: >95% by SDS-PAGE and HPLC analysis.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
• Tag: Non

Gene Information

• Gene Name: Il1f5
• Official Symbol: Il1f5
• Synonyms: IL1F5; interleukin 1 family, member 5 (delta); interleukin-36 receptor antagonist protein; IL-1F5; IL-1L1; IL-1HY1; IL-1 delta; interleukin-1 HY1; interleukin-1 delta; interleukin-1 homolog 3; interleukin-1-like protein 1; interleukin-1 family member 5; interleukin 1 receptor antagonist homolog 1; IL36RN; Il-1h3; Il1hy1; AI413231; Fil1delta
• Gene ID: 54450
• mRNA Refseq: NM_001146087
• Protein Refseq: NP_001139559
• UniProt ID: Q9QYY1

Reviews

05/31/2021

When conducting experiments with IL36RN, clear and consistent results can be obtained, facilitating data analysis and interpretation.

01/29/2019

IL36RN has high specific activity and can exert strong biological effect at low concentration.

01/11/2019

The stability of the IL36RN product allows it to cope with different experimental conditions and modes of operation.

Q&As

What is the action mechanism of IL36RN?

The mechanism of action of IL36RN is to play an anti-inflammatory, anti-infection, anti-tumor and other biological mechanisms by acting on the signaling pathway of IL-36 receptor.

How to ensure the purity of IL36RN when preparing it?

The purity of IL36RN can be ensured by ion exchange chromatography, dialysis, gel filtration and other methods.

IL36RN production process is what?

Mass production of IL36RN can be carried out through large-scale fermentation, purification and other processes.

What is the difference between IL36RN protein and IL36α, β, γ?

This protein is an inhibitor with no biological activity of its own; Recombinant IL36α, β and γ are agonists with different biological effects.

Can recombinant IL36RN be used in clinical trials and therapeutics?

Can be used in clinical trials and therapy.

Does IL36RN have sustained and stable biological activity?

IL36RN has sustained and stable biological activity.