Active Recombinant Mouse Il36a Protein (Met1-His160), C-His tagged, Animal-free, Carrier-free

• Cat.No.: Il36a-249M
• Product Overview: Recombinant Mouse Il36a Protein (Met1-His160) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Interleukin 36 alpha (IL-36 alpha) is a 17.76 kDa cytokine with 160 amino acid residues. IL-36 alpha is an IL-1 family member, which binds to the Interleukin-1 receptor and is expressed in the lung, stomach, and placenta. IL-36 alpha is critical in inflammatory responses and stimulates the production of proinflammatory cytokines, such as IL-12, IL-1β, IL-6, and TNF-α. It also induces the generation of IFNγ, IL-4, and IL-17 in CD4+ T cells.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce IL-6 secretion in 3T3 cells. The ED50 for this effect is < 15 ng/mL. The specific activity of recombinant mouse IL-36 alpha is > 1 × 10⁵ IU/mg.
• Molecular Mass: The protein has a calculated MW of 18.82 kDa.
  The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).
• Protein length: Met1-His160
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: Il36a interleukin 36A [ Mus musculus (house mouse) ]
• Official Symbol: Il36a
• Synonyms: Il36a; interleukin 36A; Fil1; If36a; Il1f6; Il1f9; IL-1H1; IL1RP2; interleukin-36 alpha; FIL1 epsilon; Fil1epsilon; IL-1 epsilon; IL-1F6; interleukin 1 family, member 6 (epsilon); interleukin 1 family, member 9; interleukin-1 epsilon; interleukin-1 family member 6; interleukin-1 homolog 1
• Gene ID: 54448
• mRNA Refseq: NM_019450
• Protein Refseq: NP_062323
• UniProt ID: Q9JLA2

Reviews

07/20/2022

When conducting experiments with HSP90AA1, clear and reliable interpretation of results can be obtained.

03/12/2021

The IL36A product has low endotoxin levels and does not interfere with experimental results.

08/12/2020

IL36A has important application value in the field of drug research and development, and has achieved certain research results.

Q&As

Does IL36A carry a fever risk?

In general, the use of IL36A does not cause significant fever reactions, but individual differences exist, if there are abnormalities, please consult a doctor.

How to prepare IL36A?

The IL36A is expressed, purified, and constructed using recombinant DNA technology in a suitable host expression system, usually through genetic engineering.

In what inflammatory diseases does recombinant IL36A have potential applications?

IL36A has potential application value in psoriasis, arthritis and other inflammatory diseases, but the specific efficacy needs further study.

Does IL36A have anti-tumor effects?

IL36A has been shown in some studies to have anti-proliferative and anti-invasive effects on certain tumors, but more studies are needed to confirm its specific mechanism of action and effect.

How is the research progress of IL36A?

The research of IL36A is progressing continuously, and some studies have shown its potential in immune regulation, anti-inflammatory effect and so on.

Can IL36A be used in animal experiments?

Yes, IL36A can be used in animal studies to investigate its mechanism of action and potential applications.