Active Recombinant Mouse Osm Protein, His-tagged

• Cat.No.: Osm-841M
• Product Overview: Recombinant mature form of mouse OSM (P53347) (Met 1-Arg 206) was expressed, with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Predicted N Terminal: Asn 25
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: 1. Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 50-200 ng/mL. 2. Measured in a cell proliferation assay using NIH3T3 cells. The ED50 for this effect is typically 0.8-4 ng/mL
• Molecular Mass: The secreted recombinant mouse OSM comprises 193 amino acids and has a calculated molecular mass of 22 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 35-40 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Osm oncostatin M [ Mus musculus ]
• Official Symbol: Osm
• Synonyms: OSM; oncostatin M; oncostatin-M; OncoM
• Gene ID: 18413
• mRNA Refseq: NM_001013365
• Protein Refseq: NP_001013383
• Pathway: Adipogenesis, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathwa
• Function: cytokine activity; cytokine activity; growth factor activity; oncostatin-M receptor binding