Species : |
Mouse |
Source : |
HEK293 |
Tag : |
mFc |
Protein Length : |
Val 20 - His 697 |
Form : |
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose. |
Bio-activity : |
Immobilized Human TfR, His Tag at 2 μg/mL (100 μL/well) can bind Mouse Transferrin Protein, Mouse IgG2a Fc Tag with a linear range of 0.4-12.5 ng/mL. Immobilized Biotinylated Cynomolgus Transferrin R, His Tag, primary amine labeling at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Mouse Transferrin Protein, Mouse IgG2a Fc Tag with a linear range of 0.3-10 ng/mL. |
Molecular Mass : |
This protein carries a mouse IgG2a Fc tag at the C-terminus.The protein has a calculated MW of 101.8 kDa. The protein migrates as 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Endotoxin : |
Less than 0.1 EU per μg by the LAL method. |
Purity : |
>95% as determined by SDS-PAGE. |
Storage : |
For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |