Active Recombinant Mouse TIGIT protein, mFc-tagged

• Cat.No.: TIGIT-0799M
• Product Overview: Recombinant Mouse TIGIT protein(Gly26-Thr143), fused with mFc tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: mFc
• Protein Length: 26-143 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Mouse CD155, Fc,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Mouse TIGIT, Mouse IgG2a Fc Tag with a linear range of 0.1-2 ng/mL.
• Molecular Mass: This protein carries a mouse IgG2a Fc tag at the C-terminus.The protein has a calculated MW of 39.8 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Gly26-Thr143
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Tigit T cell immunoreceptor with Ig and ITIM domains [ Mus musculus ]
• Official Symbol: TIGIT
• Synonyms: TIGIT; T cell immunoreceptor with Ig and ITIM domains; T-cell immunoreceptor with Ig and ITIM domains; V-set and transmembrane domain containing 3; V-set and transmembrane domain-containing protein 3; Vstm3; ENSMUSG00000071552
• Gene ID: 100043314
• mRNA Refseq: NM_001146325
• Protein Refseq: NP_001139797