Active Recombinant Pre-activated Human MMP9 Protein

• Cat.No.: MMP9-03H
• Product Overview: Recombinant human pre-activated Matrix Metalloproteinase-9 (MMP-9) is expressed in human HEK 293 cells as a glycoprotein. The DTT-reduced protein migrates as a ~67 kDa polypeptide on SDS-PAGE. This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher specific activity of the protein. The protein is produced with no artificial tags.

Specification

• Description: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
• Source: HEK293
• Species: Human
• Form: Liquid
• Bio-activity: ≥1,300 pmol/min/μg
• Molecular Mass: Calculated mol wt 66 kDa
  observed mol wt 82 kDa by SDS-PAGE (The protein migrates as a 82 kDa protein on SDS-PAGE due to glycosylation)
• Purity: ≥95% (SDS-PAGE)
• Applications: Cell analysis
• Notes: This product was pre-activated in vitro using 4-Aminophenylmercuric acetate (APMA). Thus, it is active and ready for use. In order to save our customers from handling hazardous materials, and for environmental saving, the highly toxic and fatal APMA was removed from the final preparation.
• Concentration: 30-120 μg/mL (50 μg protein, determined by Bradford. The protein concentration is 30-120 μg/mL. for the lot-specific concentration, see Certificate of Analysis.)
• Storage Buffer: Liquid solution, 0.22 mm filtered, containing 25 mM Tris, 10 mM CaCl2, 0.05% Brij-35
• Shipping: Dry ice

Gene Information

• Gene Name: MMP9 matrix metallopeptidase 9 [ Homo sapiens (human) ]
• Official Symbol: MMP9
• Synonyms: MMP9; matrix metallopeptidase 9; GELB; CLG4B; MMP-9; MANDP2; matrix metalloproteinase-9; macrophage gelatinase; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); type V collagenase; EC 3.4.24.35
• Gene ID: 4318
• mRNA Refseq: NM_004994
• Protein Refseq: NP_004985
• MIM: 120361
• UniProt ID: P14780