Active Recombinant Rabbit TGFB1 protein, His-tagged

Cat.No. : TGFB1-7844R
Product Overview : Active Recombinant Rabbit TGFB1 protein(Q4LG86)(1~99 aa), fused with N-terminal His tag, was expressed in E.coli.
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Species : Rabbit
Source : E.coli
Tag : His
Protein Length : 1-99 aa
Form : Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Bio-activity : Transforming growth factor beta 1 or TGF-β1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, and apoptosis.To test the effect of TGF-β1 on cell apoptosis, HepG2 cells were seeded into 96-well plates at a density of 4,000 cells/well with 5% serum standard DMEM including various concentrations of recombinant rabbit TGF-β1. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-2 hours at 37°C. Apoptosis of HepG2 cells after incubation with TGF-β1 for 48h observed by inverted microscope was shown. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant rabbit TGF-β1 for 48h. It was obvious thatTGF-β1 significantly inhibit cell viability of HepG2 cells. The ED50 is 7.0 μg/ml.
Molecular Mass : 14.9kDa
Endotoxin : <1.0EU per 1μg (determined by the LAL method).
Purity : > 97% as determined by SDS-PAGE.
Storage : Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
Reconstitution : Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name TGFB1 transforming growth factor, beta 1 [ Oryctolagus cuniculus ]
Official Symbol TGFB1
Synonyms TGFB1; transforming growth factor, beta 1;
Gene ID 100008645

Not For Human Consumption!

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