Active Recombinant Rabbit TGFB1 protein, His-tagged

• Cat.No.: TGFB1-7844R
• Product Overview: Active Recombinant Rabbit TGFB1 protein(Q4LG86)(1~99 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Rabbit
• Source: E.coli
• Tag: His
• Protein Length: 1-99 aa
• Form: Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Bio-activity: Transforming growth factor beta 1 or TGF-β1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, and apoptosis.To test the effect of TGF-β1 on cell apoptosis, HepG2 cells were seeded into 96-well plates at a density of 4,000 cells/well with 5% serum standard DMEM including various concentrations of recombinant rabbit TGF-β1. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-2 hours at 37°C. Apoptosis of HepG2 cells after incubation with TGF-β1 for 48h observed by inverted microscope was shown. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant rabbit TGF-β1 for 48h. It was obvious thatTGF-β1 significantly inhibit cell viability of HepG2 cells. The ED50 is 7.0 μg/ml.
• Molecular Mass: 14.9kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 97% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: TGFB1 transforming growth factor, beta 1 [ Oryctolagus cuniculus ]
• Official Symbol: TGFB1
• Synonyms: TGFB1; transforming growth factor, beta 1
• Gene ID: 100008645