Active Recombinant Bovine TGFB1 protein, His-GST-tagged

• Cat.No.: TGFB1-524C
• Product Overview: Active Recombinant Bovine TGFB1 protein(P18341)(279~390 aa), fused with N-terminal His tag and GST tag, was expressed in E.coli.

Specification

• Species: Bovine
• Source: E.coli
• Tag: GST&His
• Protein Length: 279-390 aa
• Form: Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Bio-activity: Transforming growth factor beta 1 (TGF-β1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, and apoptosis. It is reported that TGF-β1 can stimulate cell transformation by Smad signal transduction .To test the bioactivity of TGF-β1, A549 cells were seeded into 24-well plate at a density of 1x10^6 cells/mL, and allowed to attach overnight before treated with certain concentrations of TGF-β1. After 48 hours, the morphological changes of A549 were observed by inverted microscope. The results was shown. A549 cells usually have a cobblestone epithelial morphology and corresponding growth pattern. When stimulated with TGF-β1, the cells were similar with fibroblasts, the connections between cells became loose.
• Molecular Mass: 42.8kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 95% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.