Active Recombinant Rat ANGPT2 protein, His-tagged

• Cat.No.: ANGPT2-669R
• Product Overview: Recombinant Rat ANGPT2 protein(Lys 275 - Phe 496), fused with His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: Lys 275 - Phe 496
• Form: Lyophilized from 0.22 μm filtered solution in 20 mM MOPS, 150 mM NaCl, 5 mM CHAPS, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Rat Angiopoietin-2, His Tag at 2 μg/mL (100 μL/well) can bind Human TIE2, Fc Tag with a linear range of 0.039-0.313 μg/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminus.The protein has a calculated MW of 27.1 kDa. The protein migrates as 28-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Angpt2 angiopoietin 2 [ Rattus norvegicus ]
• Official Symbol: ANGPT2
• Synonyms: ANGPT2; angiopoietin 2; angiopoietin-2; Agpt2; Ang-2
• Gene ID: 89805
• mRNA Refseq: NM_134454
• Protein Refseq: NP_604449