Active Recombinant Rat Il17ra, His-tagged

• Cat.No.: Il17ra-7453R
• Product Overview: Recombinant Rat Il17ra (NP_001101353.2) (Met1-Pro320), fused with a polyhistidine tag at the C-terminus, was expressed in human cells.

Specification

• Source: Human Cells
• Species: Rat
• Tag: His
• Predicted N Terminal: Ser 32
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized rat IL17RA-His at 10 μg/ml (100 μl/well) can bind biotinylated human IL17A, The EC50 of biotinylated human IL17A is 5-11.8 ng/ml.2.Measured by its binding ability in a functional ELISA. Immobilized mouse IL17a at 10 μg/ml (100 μl/well) can bind rat IL17RA-His, The EC50 of rat IL17RA-His is 0.44-1.02 μg/mL.
• Molecular Mass: The recombinant rat IL17RA comprises 300 amino acids and predicts a molecular mass of 34.1 kDa. The apparent molecular mass of the recombinant protein is approximately 52-58 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC
• Storage: Store it under sterile conditions at -20ºC to -80ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: Il17ra interleukin 17 receptor A [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Il17ra
• Synonyms: Il17ra; interleukin 17 receptor A; Il17r; interleukin-17 receptor A
• Gene ID: 312679
• mRNA Refseq: NM_001107883
• Protein Refseq: NP_001101353
• Chromosome Location: 4q42
• Pathway: Cytokine-cytokine receptor interaction
• Function: interleukin-17 receptor activity; molecular_function