Active Recombinant Rat Pde4d Protein (2-804), N-GST tagged

• Cat.No.: Pde4d-12R
• Product Overview: Rat phosphodiesterase 4D (PDE4D) (GenBank Accession No. NM_001113328), a.a. 2-804 (end) with N-terminal GST-tag, expressed in a Baculovirus infected Sf9 cell expression system.

Specification

• Description: Enables beta-2 adrenergic receptor binding activity; cyclic-nucleotide phosphodiesterase activity; and enzyme binding activity. Involved in several processes, including positive regulation of smooth muscle cell migration; positive regulation of smooth muscle cell proliferation; and regulation of signal transduction. Located in several cellular components, including Golgi apparatus; centrosome; and myofibril. Part of protein-containing complex. Used to study mental depression. Biomarker of transient cerebral ischemia. Human ortholog(s) of this gene implicated in acrodysostosis. Orthologous to human PDE4D (phosphodiesterase 4D).
• Source: Sf9 insect cells
• Species: Rat
• Tag: N-GST
• Form: Aqueous buffer solution
• Bio-activity: ≥ 22,535 pmole/min/μg
• Molecular Mass: 117.2 kDa
• Protein Length: 2-804
• Purity: ≥80 %
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Usage: Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
• Storage: > 6 months at -80 centigrade
• Storage Buffer: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, and 20 % glycerol.
• Shipping: At -80 centigrade
• Warning: Avoid freeze/thaw cycles.

Gene Information

• Gene Name: Pde4d phosphodiesterase 4D [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Pde4d
• Synonyms: Pde4d; phosphodiesterase 4D; DPDE3; PDE3/IVd; cAMP-specific 3',5'-cyclic phosphodiesterase 4D; 3',5'-cyclic AMP phosphodiesterase; cAMP-specific phosphodiesterase PDE4D; phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila); EC 3.1.4.53
• Gene ID: 24627
• mRNA Refseq: NM_001113328
• Protein Refseq: NP_001106799
• UniProt ID: P14270