Active Recombinant Rat Vegfa

• Cat.No.: Vegfa-383R
• Product Overview: Recombinant Rat Vegfa (Accession # AAL07526.1) Ala27-Arg190 was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Rat
• Predicted N Terminal: Ala27
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323.The ED50 for this effect is typically 0.75-3.75 ng/mL.
• Molecular Mass: Recombinant Rat Vegfa has a calculated MW of 19.2 kDa (monomer). In SDS-PAGE migrates as 25 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Vegfa vascular endothelial growth factor A [ Rattus norvegicus ]
• Official Symbol: Vegfa
• Synonyms: VEGFA; vascular endothelial growth factor A; VPF; VEGF-A; vascular permeability factor; Vegf; VEGF164
• Gene ID: 83785
• mRNA Refseq: NM_001110333
• Protein Refseq: NP_001103803