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Recombinant Horse IL18 Protein, His-tagged

Cat.No. : IL18-1011H
Product Overview : Recombinant Horse IL18 Protein (Tyr37-Asn193) with His tag was expressed in E. coli.
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Description : Enables cytokine activity. Involved in lipid homeostasis; positive regulation of cell differentiation; and positive regulation of cold-induced thermogenesis. Acts upstream of or within several processes, including interleukin-18-mediated signaling pathway; positive regulation of NIK/NF-kappaB signaling; and positive regulation of interferon-gamma production. Located in extracellular space. Is expressed in several structures, including back skin; dorsal aorta; gut; lymphatic system; and reproductive system. Used to study atopic dermatitis. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Orthologous to human IL18 (interleukin 18).
Source : E. coli
Species : Horse
Tag : N-His
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 21.7 kDa; Accurate Molecular Mass: 21 kDa
Protein length : Tyr37-Asn193
Purity : > 95%
Applications : Positive Control; Immunogen; SDS-PAGE; WB.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Storage Buffer : 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Reconstitution : Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name : IL18 interleukin 18 (interferon-gamma-inducing factor) [ Equus caballus ]
Official Symbol : IL18
Synonyms : IL18; interleukin 18 (interferon-gamma-inducing factor); interleukin-18; IL-1 gamma; interleukin-1 gamma; IFN-gamma-inducing factor; interferon gamma inducing factor; interferon gamma-inducing factor; interferon-gamma-inducing factor; IGIF; IL-18;
Gene ID : 100034216
mRNA Refseq : NM_001082512
Protein Refseq : NP_001075981
UniProt ID : Q9XSQ7

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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How do researchers experimentally investigate the crosstalk between IL18 and other immune signaling pathways, such as Toll-like receptor signaling? 10/28/2022

Co-immunoprecipitation, co-localization studies, and pathway inhibition assays decipher IL18's interactions with signaling molecules and pathways.

What assays and methodologies are employed to evaluate the potential therapeutic interventions targeting IL18 in autoimmune diseases and chronic inflammation? 03/23/2022

Therapeutic antibody trials, in vitro drug screens, and disease model assessments assess IL18-targeted interventions in autoimmune diseases.

How are animal models and in vivo imaging techniques used to understand the kinetics of IL18-mediated immune responses and its tissue-specific effects? 01/10/2022

Xenograft models, intravital imaging, and tissue-specific knockout approaches unravel IL18's kinetics and effects within complex in vivo environments.

Can you elaborate on the techniques used to assess the impact of IL18 on immune cell activation, migration, and effector functions? 06/11/2021

Flow cytometry, chemotaxis assays, and functional cell assays examine IL18's impact on immune cell activation, migration, and effector functions.

Can you explain the experimental strategies used to analyze the interactions between IL18 and its receptor complex, as well as the downstream signaling events triggered by receptor engagement? 01/12/2021

Surface plasmon resonance, mutational studies, and phosphoproteomics illuminate IL18's receptor interactions and downstream signal transduction pathways.

How are cellular and molecular techniques employed to investigate IL18's role in regulating the balance between Th1 and Th2 immune responses? 08/01/2020

Immunophenotyping, cytokine profiling, and gene expression analyses elucidate IL18's influence on Th cell polarization and immune balance.

What experimental approaches are utilized to study the genetic and epigenetic regulation of IL18 expression in response to different stimuli? 04/29/2020

ChIP assays, promoter analyses, and epigenetic profiling uncover the regulatory mechanisms underlying IL18 gene expression dynamics.

What methods are used to dissect the mechanisms through which IL18 contributes to the pathogenesis of inflammatory diseases and its interactions with other proinflammatory molecules? 12/15/2018

Disease models, knockout mice, and co-stimulation studies explore IL18's involvement in inflammatory conditions and its interplay with other proinflammatory mediators.

How do researchers investigate the role of IL18 in modulating the tumor microenvironment and its potential implications for cancer immunotherapy? 11/05/2018

Tumor models, immune profiling, and immunotherapeutic studies investigate IL18's modulation of the tumor microenvironment and its implications for cancer therapy.

Could you explain the experimental strategies used to characterize the inflammasome activation triggered by IL18 and its downstream effects on cytokine production? 04/20/2018

Caspase assays, cytokine ELISAs, and microscopy techniques unveil the activation of inflammasomes and IL18's contribution to pyroptosis and cytokine storms.

Customer Reviews (3)

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Reviews
10/15/2022

    Effective in inflammation models.

    08/22/2021

      High stability in serum.

      05/09/2019

        Suitable for ELISA.

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