Recombinant Human KDSR protein, His-tagged

• Cat.No.: KDSR-3461H
• Product Overview: Recombinant Human KDSR(26-270aa) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: KDSR, also known as 3-ketodihydrosphingosine reductase, is a 332 amino acid multi-pass membrane protein that localizes to the endoplasmic reticulum (ER) and belongs to the short-chain dehydrogenases/reductases (SDR) family. KDSR is a secreted protein which is weakly expressed in hematopoietic tissue. Also this protein catalyzes the reduction of 3-ketodihydrosphingosine (KDS) to dihydrosphingosine (DHS).
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl, 0.1mM PMSF
• Molecular Mass: 29.0 kDa (266aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MSQDYNQVENV IKQAQEKLGP VDMLVNCAGM AVSGKFEDLE VSTFERLMSI NYLGSVYPSR AVITTMKERR VGRIVFVSSQ AGQLGLFGFTKPLALPGAH VVVTGGSSGI GKCIAIECYK QGAFITLVAR NEDKLLQAKK EIEMHSINDK QVVLCISVDVAYSASKFAIR GLAEALQMEV KPYNVYITVA YPPDTDTPGF AEENRTKPLE TRLISETTSV CKPEQVAKQI VKDAIQGNFN SSLGSD
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: KDSR 3-ketodihydrosphingosine reductase [ Homo sapiens ]
• Official Symbol: KDSR
• Synonyms: KDSR; 3-ketodihydrosphingosine reductase; follicular lymphoma variant translocation 1 , FVT1; 3 dehydrosphinganine reductase; DHSR; SDR35C1; short chain dehydrogenase/reductase family 35C; member 1; FVT-1; KDS reductase; 3-dehydrosphinganine reductase; follicular variant translocation protein 1; follicular lymphoma variant translocation 1; short chain dehydrogenase/reductase family 35C, member 1; FVT1; FLJ36555; FLJ92680
• Gene ID: 2531
• mRNA Refseq: NM_002035
• Protein Refseq: NP_002026
• MIM: 136440
• UniProt ID: Q06136
• Chromosome Location: 18q21
• Pathway: Ceramide biosynthesis, organism-specific biosystem; Ceramide biosynthesis, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Sphingolipid de novo biosynthesis, organism-specific biosystem; Sphingolipid metabolism, organism-specific biosystem
• Function: 3-dehydrosphinganine reductase activity; nucleotide binding; oxidoreductase activity