"BCL2" Related Products

Recombinant Human B-Cell CLL/Lymphoma 2, His-tagged

Cat. No.: BCL2-5289H
Product Overview: Recombinant human BCL2with His Tag was expressed in E. coli.
Description: Recombinantfull length human BCL2 is a single, non-glycosylated polypeptide chaincontaining amino acids 1-206. The wild type BCL2 is missing 12 amino acidsfrom the C-terminus. The C-terminus is fused to a His-Tag. The C-terminushis-tag mimics the deleted C-terminus membrane domain thus maintaining itsbiological activity.
Source: E. coli
Species: Human
Form: Lyophilized from asterile filtered solution containing 10 mM Tris-HCl, pH 8.0 + 1 mM EDTA + 250mM NaCl.
Purity: > 95% asdetermined by RP-HPLC and SDS-PAGE analyses.
Endotoxin Level: < 0.1 ng/µg ofBCL2
Reconstitution: Centrifuge vialprior to opening. Suspend BCL2 in 100 µl of 0.5M Acetic acid, overnight at 2-4°C. Dilute 10 fold into selected buffer system. Because BCL2 hasa tendency to form intramolecular disulfide bond, 5 mM DTT is recommended inassay buffer. When running SDS-PAGE gel, 10 mM DTT is recommended.
Storage: Store lyophilizedprotein at -20°C to -80°C. Reconstituted protein is stable for 1 week at2-4°C. For long term storage, aliquot and store at -20°C to -80°C with acarrier protein (0.1% HSA or BSA) as a stabilizer. Please note that theaddition of any carrier protein into this product may produce unwantedendotoxin. This depends upon the particular application employed. Avoidrepeated freeze-thaw cycles.
OfficialSymbol: BCL2
Gene Name: BCL2 B-cell CLL/lymphoma 2 [ Homosapiens ]
Synonyms: BCL2; B-cellCLL/lymphoma 2; Bcl-2; PPP1R50; apoptosisregulator Bcl-2; protein phosphatase 1, regulatory subunit 50
Gene ID: 596
mRNA Refseq: NM_000633
Protein Refseq: NP_000624
MIM: 151430
UniProt ID: P10415
Chromosome Location: 18q21.33; 18q21.3
Pathway: ATF-2 transcriptionfactor network; Activation of BAD and translocation to mitochondria;Activation of BH3-only proteins; Amyotrophic lateral sclerosis (ALS);Apoptosis; B Cell Receptor Signaling Pathway; BH3-only proteins associatewith and inactivate anti-apoptotic BCL-2 members; Caspase cascade inapoptosis; Cholinergic synapse; Colorectal cancer; RXR and RARheterodimerization with other nuclear receptor; Small cell lung cancer; TheNLRP1 inflammasome; Toxoplasmosis; Tuberculosis
Function: BH3 domain binding;channel activity; identical protein binding; protease binding; proteinbinding; protein heterodimerization activity; protein homodimerizationactivity; protein phosphatase 2A binding; sequence-specific DNA binding;transcription factor binding; ubiquitin protein ligase binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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