Recombinant Human B-Cell CLL/Lymphoma 2, His-tagged


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Recombinant Human B-Cell CLL/Lymphoma 2, His-tagged

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Cat. No. : BCL2-5289H
Product Overview : Recombinant human BCL2with His Tag was expressed in E. coli.
Description : Recombinantfull length human BCL2 is a single, non-glycosylated polypeptide chaincontaining amino acids 1-206. The wild type BCL2 is missing 12 amino acidsfrom the C-terminus. The C-terminus is fused to a His-Tag. The C-terminushis-tag mimics the deleted C-terminus membrane domain thus maintaining itsbiological activity.
Source : E. coli
Species : Human
Form : Lyophilized from asterile filtered solution containing 10 mM Tris-HCl, pH 8.0 + 1 mM EDTA + 250mM NaCl.
Purity : > 95% asdetermined by RP-HPLC and SDS-PAGE analyses.
Endotoxin Level : < 0.1 ng/µg ofBCL2
Reconstitution : Centrifuge vialprior to opening. Suspend BCL2 in 100 µl of 0.5M Acetic acid, overnight at 2-4°C. Dilute 10 fold into selected buffer system. Because BCL2 hasa tendency to form intramolecular disulfide bond, 5 mM DTT is recommended inassay buffer. When running SDS-PAGE gel, 10 mM DTT is recommended.
Storage : Store lyophilizedprotein at -20°C to -80°C. Reconstituted protein is stable for 1 week at2-4°C. For long term storage, aliquot and store at -20°C to -80°C with acarrier protein (0.1% HSA or BSA) as a stabilizer. Please note that theaddition of any carrier protein into this product may produce unwantedendotoxin. This depends upon the particular application employed. Avoidrepeated freeze-thaw cycles.
OfficialSymbol : BCL2
PDB rendering basedon 1GJH, 1G5M. :

Gene Name : BCL2 B-cell CLL/lymphoma 2 [ Homosapiens ]
Synonyms : BCL2; B-cellCLL/lymphoma 2; Bcl-2; PPP1R50; apoptosisregulator Bcl-2; protein phosphatase 1, regulatory subunit 50
Gene ID : 596
mRNA Refseq : NM_000633
Protein Refseq : NP_000624
MIM : 151430
UniProt ID : P10415
Chromosome Location : 18q21.33; 18q21.3
Pathway : ATF-2 transcriptionfactor network; Activation of BAD and translocation to mitochondria;Activation of BH3-only proteins; Amyotrophic lateral sclerosis (ALS);Apoptosis; B Cell Receptor Signaling Pathway; BH3-only proteins associatewith and inactivate anti-apoptotic BCL-2 members; Caspase cascade inapoptosis; Cholinergic synapse; Colorectal cancer; RXR and RARheterodimerization with other nuclear receptor; Small cell lung cancer; TheNLRP1 inflammasome; Toxoplasmosis; Tuberculosis
Function : BH3 domain binding;channel activity; identical protein binding; protease binding; proteinbinding; protein heterodimerization activity; protein homodimerizationactivity; protein phosphatase 2A binding; sequence-specific DNA binding;transcription factor binding; ubiquitin protein ligase binding
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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