||Recombinant Swine TNF-α was expressed inYeast. MW= 16.9 kDa (154 aa).
||TNF-alpha is a homotrimer with a subunit molecular mass of 17 kDa and that it plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases; and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF was found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease.
||Lyophilized. Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein.
||>95% as visualized by SDS-PAGE analysis.
|Amino Acid Sequence:
||SSSQTSDKPV AHVVANVKAE GQLQWQSGYA NALLANGVKL KDNQLVVPTD GLYLIYSQVL FRGQGCPSTN VFLTHTISRI AVSYQTKVNL LSAIKSPCQR ETPEGAEAKP WYEPIYLGGV FQLEKDDRLS AEINLPDYLD FAESGQVYFG IIAL.
||The biological activity of recombinant swine TNF alpha was measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells. The ED50 for this effect is typically 1 3 ng/mL.
|Stability And Storage:
||Stable for up to twelve months from date of receipt at -20°C. Stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freeze/thaw cycles.