Recombinant Human Colony Stimulating Factor 1 (macrophage)

• Cat.No.: CSF1-357H
• Product Overview: Recombinanthuman CSF1is a single, non-glycosylated polypeptide chain containing 150 amino acids,MW 42.0 kDa.CSF1is expressed in SF9 cells, MW 42.0 kDa.

Specification

• Cat. No.: CSF1-357H
• Description: Macrophagecolony-stimulating factor, or CSF1, is a secreted cytokine which influenceshematopoietic stem cells to differentiate into macrophages or other relatedcell types. Eukaryotic cells also produce CSF1 in order to combat intercellularviral infection. (See colony-stimulating factor.) CSF1 binds to the Colonystimulating factor 1 receptor. It may also be involved in development of theplacenta.
• Source: Human
• Host: SF9 cells
• Form: Lyophilizedfrom a 0.2 μm filtered solution in 20 mM PB + 1% HSA + 3% Mannitol.
• Purity: >95% by SDS-PAGEand HPLC.
• Sequence: EEVSEYCSHMIGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQLQELSLRLKSC FTKDYEEHDKACVRTFYETP LQLLEKVKNV FNETKNLLDK DWNIFSKNCN NSFAECSSQ
• Molecular Weight: 42.0 kDa
• Biological Activity: Fullybiologically active when compared to standard. Determined by thedose-dependant stimulation of the proliferation of murine M-NSF-60 cells. TheED is <3.0 ng/Ml.
• Endotoxin Level: <1EU/µg of MCSF.
• Reconstitution: Centrifugevial prior to opening. Add sterile distilled water or aqueous buffer containing0.1% BSA to a concentration of 0.1-1.0 mg/mL. Please note that the additionof any carrier protein into this product may produce unwanted endotoxin. Thisdepends upon the particular application employed. Further dilutions should bemade in appropriate buffered solutions.
• Storage: Stableat 2-8°C, but best kept desiccated -20°C. Upon reconstitution, stable for upto 1 week at 2-8°C. For longer term, store in working aliquots below -20°C. Avoidrepeated freeze/thaw cycles.
• OfficialSymbol: CSF1

Gene Information

• Gene Name: CSF1colony stimulating factor 1 (macrophage) [ Homo sapiens ]
• Synonyms: CSF1; colonystimulating factor 1 (macrophage); MCSF; M-CSF; CSF-1; MGC31930; macrophagecolony stimulating factor; macrophage colony-stimulating factor 1;Lanimostim; lanimostim; OTTHUMP00000013362; OTTHUMP00000013363;OTTHUMP00000013364; OTTHUMP0-0000013365; EC 2.7.10.1
• Gene ID: 1435
• mRNA Refseq: NM_000757
• Protein Refseq: NP_000748
• MIM: 120420
• UniProt ID: P09603
• Chromosome Location: 1p21-p13
• Pathway: Cytokine-cytokinereceptor interaction; Cytokines and Inflammatory Response; Hematopoietic celllineage; Integrins in angiogenesis; Osteoclast differentiation; Rheumatoidarthritis; Signaling events mediated by PTP1B; Signaling events mediated byTCPTP
• Function: cytokineactivity; growth factor activity; macrophage colony-stimulating factorreceptor binding; protein homodimerization activity