| Cat. No. : |
Csf2-370M |
| Description : |
Csf2 is amonomeric protein of 127 amino acids with two glycosylation sites. Theprotein is synthesized as a precursor of 144 amino acids, which included ahydrophobic secretory signal sequence at the aminoterminal end. The sugarmoiety is not required for the full spectrum of biological activities. Non-glycosylated and glycosylated Csf2 show the same activities in vitro. Fullyglycosylated Csf2 is biologically more active in vivo than the non-glycosylated protein. This protein is secreted together with other factors byT-cells and macrophages following cell activation by antigens or mitogens. |
| Source : |
Mouse |
| Host : |
E. coli |
| Purity : |
>95%, asdetermined by SDS-PAGE and HPLC. |
| Sequence : |
MWLQNLLFLGIVVYSLSAPT RSPITVTRPW KHVEAIKEAL NLLDDMPVTL NEEVEVVSNE FSFKKLTCVQ TRLKIFEQGLRGNFTKLKGA LNMTASYYQT YCPPTPETDC ETQVTTYADF IDSLKTFLTD IPFECKKPGQ K |
| Molecular Weight : |
Native MouseGranulocyte Macrophage-Colony Stimulating Factor is generated by the proteolyticremoval of the signal peptide and propeptide, this molecule has a calculated massof 14 kDa. |
| Biological Activity : |
The ED50 asdetermined by the dose-dependent stimulation of the proliferation of murineFDC-P1 cells is ≤ 0.1ng/ml, corresponding to a specific activity of ≥ 3 x 106units/mg. |
| Endotoxin : |
Endotoxincontent was assayed using a LAL gel clot method. Endotoxin level was foundto be less than 0.1 ng/µg(1EU/µg). |
| Reconstitution : |
A quick spin of thevial followed by reconstitution in distilled water to a concentration notless than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage : |
Thelyophilized protein is stable for at least 2 years from date of receipt at-20°C. Upon reconstitution, this cytokine can be stored in working aliquots at2°C - 8°C for one month, or at -20°C for six months, with a carrier proteinwithout detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| OfficialSymbol : |
Csf2 |
| Pathways : |
Amoebiasis;Cytokine Signaling in Immune system; Cytokine-cytokine receptor interaction; Fcepsilon RI signaling pathway; HTLV-I infection; Hematopoietic cell lineage; ImmuneSystem; Interleukin receptor SHC signaling; Interleukin-2 signaling; Jak-STATsignaling pathway; Natural killer cell mediated cytotoxicity; Rheumatoidarthritis; Salmonella infection; Signaling by Interleukins; T cell receptorsignaling pathway |
