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Recombinant Mouse Colony Stimulating Factor 2 (granulocyte-macrophage)

Cat. No.: Csf2-370M
Product Overview: Recombinantmouse Csf2 is a disulfide-linked monomer protein, consisting of 125 aminoacids and migrates as an approximately 14 kDa protein under reducingconditions, and was lyophilized from 0.2 μm filtered PBSsolution, pH 7.0.
Description: Csf2 is amonomeric protein of 127 amino acids with two glycosylation sites. Theprotein is synthesized as a precursor of 144 amino acids, which included ahydrophobic secretory signal sequence at the aminoterminal end. The sugarmoiety is not required for the full spectrum of biological activities. Non-glycosylated and glycosylated Csf2 show the same activities in vitro. Fullyglycosylated Csf2 is biologically more active in vivo than the non-glycosylated protein. This protein is secreted together with other factors byT-cells and macrophages following cell activation by antigens or mitogens.
Source: Mouse
Host: E. coli
Purity: >95%, asdetermined by SDS-PAGE and HPLC.
Sequence: MWLQNLLFLGIVVYSLSAPT RSPITVTRPW KHVEAIKEAL NLLDDMPVTL NEEVEVVSNE FSFKKLTCVQ TRLKIFEQGLRGNFTKLKGA LNMTASYYQT YCPPTPETDC ETQVTTYADF IDSLKTFLTD IPFECKKPGQ K
Molecular Weight: Native MouseGranulocyte Macrophage-Colony Stimulating Factor is generated by the proteolyticremoval of the signal peptide and propeptide, this molecule has a calculated massof 14 kDa.
Biological Activity: The ED50 asdetermined by the dose-dependent stimulation of the proliferation of murineFDC-P1 cells is ≤ 0.1ng/ml, corresponding to a specific activity of ≥ 3 x 106units/mg.
Endotoxin: Endotoxincontent was assayed using a LAL gel clot method. Endotoxin level was foundto be less than 0.1 ng/µg(1EU/µg).
Reconstitution: A quick spin of thevial followed by reconstitution in distilled water to a concentration notless than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage: Thelyophilized protein is stable for at least 2 years from date of receipt at-20°C. Upon reconstitution, this cytokine can be stored in working aliquots at2°C - 8°C for one month, or at -20°C for six months, with a carrier proteinwithout detectable loss of activity. Avoid repeated freeze/thaw cycles.
OfficialSymbol: Csf2
Pathways: Amoebiasis;Cytokine Signaling in Immune system; Cytokine-cytokine receptor interaction; Fcepsilon RI signaling pathway; HTLV-I infection; Hematopoietic cell lineage; ImmuneSystem; Interleukin receptor SHC signaling; Interleukin-2 signaling; Jak-STATsignaling pathway; Natural killer cell mediated cytotoxicity; Rheumatoidarthritis; Salmonella infection; Signaling by Interleukins; T cell receptorsignaling pathway
Gene Name: Csf2 colony stimulating factor 2 (granulocyte-macrophage)[ Mus musculus ]
Synonyms: Csf2; colony stimulating factor 2 (granulocyte-macrophage);Csfgm;Gm-CSf; MGI-IGM; MGC151255; MGC151257; granulocyte-macrophagecolony-stimulating factor; CSF; put. GM-CSF; OTTMUSP00000005830;colony-stimulating factor; granulocyte-macrophage colony stimulating factor 2
Gene ID: 12981
mRNA Refseq: NM_009969
Protein Refseq: NP_034099
UniProt ID: P01587
Function: cytokineactivity; granulocyte macrophage colony-stimulating factor receptor binding; growthfactor activity

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