Recombinant Human Protein Tyrosine Phosphatase, Non-Receptor Type 1, GST-tagged

• Cat.No.: PTPN1-1908H
• Product Overview: RecombinantHuman PTPN1, full length, (aa 1-436) with N-terminal GST tag.

Specification

• Cat. No.: PTPN1-1908H
• Description: PTPN1 (PTP1B) isan enzyme that is the founding member of the protein tyrosine phosphatase(PTP) family. In humans it is encoded by the PTPN1 gene. PTPN1 is anegative regulator of the insulin signaling pathway and is considered apromising potential therapeutic target, in particular for treatment of type 2diabetes.
• Source: E. coli
• Species: Human
• Form: Liquid in 25 mM Tris-HCl, pH 8.0 +100 mM NaCl + 0.05% Tween-20 + 50% glycerol + 3 mM DTT.
• Molecular Weight: 76kDa
• Purity: > 70%
• Specific Activity: 10 U/ug.One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.Assay buffer: 50 mM HEPES, pH 7.4 + 2 mM EDTA + 3mM DTT + 100 mM NaCl + 50mM Pnpp.
• Applications: Useful for the study of enzymekinetics, regulation, to dephosphorylate target substrates and forscreening inhibitors.
• Storage: Stablefor 6 months at -80°C. Avoid repeated freeze-thaw cycles.
• Pathways: Adherensjunction; Calcineurin-regulated NFAT-dependent transcription in lymphocytes;Cytokine Signaling in Immune system; EGF receptor (ErbB1) signaling pathway;Growth hormone receptor signaling; Hemostasis; IGF1 pathway; Immune System;Insulin Pathway; Insulin signaling pathway; Integrin alphaIIb beta3signaling; Interferon alpha/beta signaling; N-cadherin signaling events;PDGFR-beta signaling pathway; Regulation of IFNA signaling

Gene Information

• Gene Name: PTPN1protein tyrosine phosphatase, non-receptor type 1 [ Homo sapiens ]
• Official Symbol: PTPN1
• Synonyms: PTPN1;protein tyrosine phosphatase, non-receptor type 1; PTP1B; tyrosine-proteinphosphatase non-receptor type 1; OTTHUMP00000031266; protein tyrosinephosphatase 1B; protein-tyrosine phosphatase 1B; protein tyrosine phosphatase,placental
• Gene ID: 5770
• mRNA Refseq: NM_002827
• Protein Refseq: NP_002818
• MIM: 176885
• UniProt ID: P18031
• Chromosome Location: 20q13.1-q13.2
• Function: ephrinreceptor binding; hydrolase activity; insulin receptor binding; proteinbinding; protein tyrosine phosphatase activity; zinc ion binding

Reviews

04/06/2023

Timely and precise. Essential for achieving research goals.

08/30/2020

Efficient service, valuable insights. Key to research success.

03/26/2018

Saves time and resources. Critical for our research.

Q&As

What is the impact of altered PTPN1 activity on obesity and related metabolic disorders?

Altered PTPN1 activity is associated with obesity, as it impacts pathways involved in energy balance and fat storage.

How do genetic variations in PTPN1 contribute to metabolic disease susceptibility?

Genetic variations in PTPN1 can affect its activity and thereby influence metabolic processes and disease risk.

What role does PTPN1 play in the development of insulin resistance and type 2 diabetes?

PTPN1 is implicated in insulin resistance, potentially contributing to the development of type 2 diabetes.

How does PTPN1 regulate insulin signaling and glucose metabolism?

It negatively regulates insulin signaling by dephosphorylating insulin receptor substrates, affecting glucose uptake and metabolism.

How does PTPN1 interact with other signaling molecules in regulating metabolic pathways?

PTPN1 interacts with various signaling proteins, playing a role in diverse metabolic pathways including lipid and glucose metabolism.

What is the primary function of PTPN1 in cellular signaling?

PTPN1, also known as Protein Tyrosine Phosphatase Non-Receptor Type 1, is key in dephosphorylating proteins involved in signal transduction.

What potential therapeutic applications could arise from targeting PTPN1 in metabolic diseases?

Targeting PTPN1 could offer therapeutic benefits in treating insulin resistance, type 2 diabetes, and obesity.