||Recombinant human full length MAP4K5,GST-tagged is expressed in Insect cells.
||MAP4K5is an enzyme that in humans is encoded by the MAP4K5 gene. This gene encodesa member of the serine/threonine protein kinase family that is highly similarto yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning ofthe MAP kinase signal cascades that is essential for yeast pheromoneresponse. This kinase was shown to activate Jun kinase in mammalian cells,which suggested a role in stress response. Two alternatively splicedtranscript variants encoding the same protein have been described for thisgene.
||Liquid in50 mM Tris, pH 7.5 + 150 mM NaCl + 0.5 mM EDTA + 0.02% Triton X-100 + 2 mMDTT + 50% glycerol.
||780 nmole of phosphate transferred tomyelin basic protein (MBP) per minute per mg of total protein at 30°C.Activity determined at a final protein concentration of 0.83 μg/ml.
||Stablefor 6 months in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.
|Function||ATPbinding; nucleotide binding; protein serine/tyreonine kinase activity; protein binding;small GTPase regulator activity; protein kinase activity|