||Recombinant human S100A7 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
||S100A7, also known as Psoriasin, belongs to the S-100 family of calcium binding proteins and is secreted via a non-classical secretory pathway into the cytoplasm. It is thought to function in the regulation of many cellular processes, including the cell cycle, cell progression and cellular differentiation. It contains two EF-hand domains and is highly upregulated in psoriatic epidermis, as well as in bladder squamous cell carcinoma and breast cancer tissue, suggesting a possible role in carcinogenesis.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 30% glycerol; 0.1M NaCl, 1mM DTT
||13.6 kDa (121aa), confirmed by MALDI-TOF
||> 95% by SDS - PAGE
||1.0 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSHMSNTQAERSI IGMIDMFHKY TRRDDKIDKP SLLTMMKENFPNFLSACDKK GTNYLADVFE KKDKNEDKKIDFSEFLSLLG DIATDYHKQS HGAAPCSGGSQ
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.