"FZD8" Related Products

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Recombinant Human Frizzled Family Receptor 8, Fc-tagged

Cat. No.: FZD8-597H
Product Overview: RecombinantHuman FZD8 protein was expressed in Chinese Hamster Ovary cell line with theFc part of Human IgG1 Pro100-Lys330 (C-terminus) and Human Frizzled-8 Ala25-Pro172(N-terminus).
Description: FZD8 is one of atleast ten seventransmembrane (7TM) glycoprotein of the Frizzled family of Wntreceptors. Frizzled proteins are thought to be G-protein-coupled. Wnt engagement,with low density lipoprotein receptor related proteins LRP-5 or LRP-6 actingas co-receptors stabilizes β-catenin and promotes gene transcription that isimportant in development and tissue maintenance.
Source: Chinese HamsterOvary cell line, CHO-derived
Form: Supplied as a 0.2 μmfiltered solution in PBS.
N-terminal Sequence: Ala25 & Ala28
Molecular Weight: 43.3 kDa
Activity: Measured by itsbinding ability in a functional ELISA. In a 100 μL reaction mixturecontaining biotinylated rmWnt-3a or rmWnt-5a at 50-100 ng/mL and rhFrizzled8/Fc Chimera dilutions at 0.1-8,000 ng/mL, the concentration of rhFrizzled8/ FcChimera that produces 50% of the optimal binding response is found to beapproximately 7-28 ng/mL.
Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
SDS-PAGE: 55-65 kDa, reducingconditions
Purity: >95%, by SDS-PAGEunder reducing conditions and visualized by silver stain.
Reconstitution: Reconstitute at 500μg/mL in sterile PBS.
Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to-70 °C under sterile conditions after reconstitution.
Gene Name: FZD8 frizzled family receptor 8[ Homo sapiens ]
Official Symbol: FZD8
Synonyms: FZD8; frizzledfamily receptor 8; FZ-8; hFZ8; frizzled (Drosophila) homolog 8;frizzled 8, seven transmembrane spanning receptor; frizzled homolog 8(Drosophila); OTTHUMP00000019454; hFz8; Fz-8; frizzled-8; frizzledhomolog 8
Gene ID: 8325
mRNA Refseq: NM_031866
Protein Refseq: NP_114072
MIM: 606146
UniProt ID: Q9H461
Chromosome Location: 10p11.2
Pathway: Basal cellcarcinoma; Class B/2 (Secretin family receptors); GPCR ligand binding; HTLV-Iinfection; Melanogenesis; Pathways in cancer; Signal Transduction; Signalingby GPCR; Wnt Signaling Pathway; Wnt signaling network
Function: G-proteincoupled receptor activity; PDZ domain binding; Wnt-activated receptoractivity; Wnt-protein binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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