| Cat. No. : |
Il23a-651M |
| Description : |
Il23a isa heterodimeric cytokine composed of two disulfide-linked subunits, a p19subunit that is unique to Il23a, and a p40 subunit that is shared with IL-12.The Il23a homology to the p35 subunit of IL-12, as well as to other singlechain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to theextracellular domains of the hematopoietic cytokine receptors. Mouse p19 cDNAencodes a 196 amino acid residue (aa) precursor protein with a putative 19 aasignal peptide and 177 aa mature protein. Human and mouse Il23a share 70% aasequence identity. Although p19 is expressed by activated macrophages,dendritic cells, T cells, and endothelial cells, only activated macrophagesand dendritic cells express p40 concurrently to produce Il23a. |
| Source : |
Spodoptera frugiperda, Sf 21(baculovirus)-derived. |
| Form : |
Lyophilizedfrom a 0.2 um filtered solution in PBS with BSA as a carrier protein. |
| N-terminalSequence : |
Met23 |
| MolecularWeight : |
57 kDa |
| Activity : |
Measuredby its ability to induce IL-17 secretion by mouse splenocytes. The ED 50 for thiseffect is typically 0.05-0.25 ng/mL. |
| Endotoxin Level : |
<1.0EU per 1μg of the protein by the LAL method. |
| SDS-PAGE : |
66 kDa,reducing conditions. |
| Purity : |
>90%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Reconstitution : |
Reconstituteat 10μg/mL in sterile PBS containing at least 0.1% human or bovine serumalbumin. |
| Storage : |
Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution. |
