||Recombinant humanAKAP7 protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography techniques.
||AKAP7 (A-kinaseanchor protein 7 isoform gamma isoform alpha) , also known as AKAP18, belongsto the A-kinase anchoring protein (AKAP) family, a group of functionallyrelated proteins that bind to a regulatory subunit (RII) of cAMP-dependentprotein kinase A (PKA) and target the enzyme to specific subcellularcompartments. AKAP 7 is expressed in brain, heart, lung, pancreas andskeletal muscle. AKAP 7 binds PKA to the plasma membrane, and permitsfunctional coupling to the L-type calcium channel
||Liquid. In 20 mMTris-HCl buffer, pH8.0, 10% glycerol, 1mM DTT, 50mM NaCl
||11.5kDa (105aa),confirmed by MALDI-TOF
||> 90% by SDS -PAGE
||1mg/ml (determinedby Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMGQLCC FPFSRDEGKI SEKNGGEPDD AELVRLSKRL VENAVLKAVQ QYLEETQNKNKPGEGSSVKT EAADQNGNDN ENNRK
||Stored at +4°C shortterm (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.